Remarkably high activities of testicular cytochrome c in destroying reactive oxygen species and in triggering apoptosis
文献类型：期刊论文
| 作者 | Liu, Zhe; Lin, Hao; Ye, Sheng; Liu, Qin-Ying; Meng, Zhaohui; Zhang, Chuan-Mao; Xia, Yongjing; Margoliash, Emanuel; Rao, Zihe; Liu, Xiang-Jun |
| 刊名 | Proceedings of the national academy of sciences of the united states of america
 |
| 出版日期 | 2006-06-13 |
| 卷号 | 103期号:24页码:8965-8970 |
| 关键词 | Mouse testis Antioxidation |
| ISSN号 | 0027-8424 |
| DOI | 10.1073/pnas.0603327103 |
| 通讯作者 | Margoliash, emanuel(maem@uic.edu) |
| 英文摘要 | Hydrogen peroxide (h(2)o(2)) is the major reactive oxygen species (ros) produced in sperm. high concentrations of h(2)o(2) in sperm induce nuclear dna fragmentation and lipid peroxidation and result in cell death. the respiratory chain of the mitochondrion is one of the most productive ros generating systems in sperm, and thus the destruction of ros in mitochondria is critical for the cell. it was recently reported that h(2)o(2) generated by the respiratory chain of the mitochondrion can be efficiently destroyed by the cytochrome c-mediated electron-leak pathway where the electron of ferrocytochrome c migrates directly to h(2)o(2) instead of to cytochrome c oxidase. in our studies, we found that mouse testis-specific cytochrome c (t-cc) can catalyze the reduction of h(2)o(2) three times faster than its counterpart in somatic cells (s-cc) and that the t-cc heme has the greater resistance to being degraded by h(2)o(2). together, these findings strongly imply that t-cc can protect sperm from the damages caused by h(2)o(2). moreover, the apoptotic activity of t-cc is three to five times greater than that of s-cc in a well established apoptosis measurement system using xenopus egg extract. the dramatically stronger apoptotic activity of t-cc might be important for the suicide of male germ cells, considered a physiological mechanism that regulates the number of sperm produced and eliminates those with damaged dna. thus, it is very likely that t-cc has evolved to guarantee the biological integrity of sperm produced in mammalian testis. |
| WOS关键词 | OXIDATIVE STRESS ; HYDROGEN-PEROXIDE ; HUMAN-SPERMATOZOA ; MALE-INFERTILITY ; MITOCHONDRIA ; GENERATION ; SPERM ; SITE ; INITIATION ; DROSOPHILA |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000238369100014 |
| 出版者 | NATL ACAD SCIENCES |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379838 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Margoliash, Emanuel |
| 作者单位 | 1.Northwestern Univ, Dept Biochem Mol Biol & Cell Biol, Evanston, IL 60208 USA 2.Tsinghua Univ, Inst Biomed Informat, Sch Med, Beijing 100084, Peoples R China 3.Tsinghua Univ, MOE Lab Prot Sci, Beijing 100084, Peoples R China 4.Tsinghua Univ, Struct Biol Lab, Beijing 100084, Peoples R China 5.Peking Univ, Coll Life Sci, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Zhe,Lin, Hao,Ye, Sheng,et al. Remarkably high activities of testicular cytochrome c in destroying reactive oxygen species and in triggering apoptosis[J]. Proceedings of the national academy of sciences of the united states of america,2006,103(24):8965-8970. |
| APA | Liu, Zhe.,Lin, Hao.,Ye, Sheng.,Liu, Qin-Ying.,Meng, Zhaohui.,...&Liu, Xiang-Jun.(2006).Remarkably high activities of testicular cytochrome c in destroying reactive oxygen species and in triggering apoptosis.Proceedings of the national academy of sciences of the united states of america,103(24),8965-8970. |
| MLA | Liu, Zhe,et al."Remarkably high activities of testicular cytochrome c in destroying reactive oxygen species and in triggering apoptosis".Proceedings of the national academy of sciences of the united states of america 103.24(2006):8965-8970. |
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来源：中国科学院大学
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