New method for hplc separation and fluorescence detection of malonaldehyde in normal human plasma
文献类型：期刊论文
| 作者 | Mao, JJ; Zhang, HW; Luo, J; Li, L; Zhao, R; Zhang, RN; Liu, GQ |
| 刊名 | Journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2006-02-17 |
| 卷号 | 832期号:1页码:103-108 |
| 关键词 | Malonaldehyde 9-fluorenylmethoxycarbonyl hydrazine Plasma Fluorescence detection |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2005.12.041 |
| 通讯作者 | Liu, gq() |
| 英文摘要 | A new method for the detection of free and total malonaldehyde (mda) in human plasma samples based on the derivatization of mda with 9-fluorenylmethoxycarbonyl hydrazine (fmoc-hydrazine) in an acidic medium was developed. derivatization was achieved after 4h at 50 degrees c. the derivatized samples were analyzed by hplc using a reversed-phase c-18 column with fluorescence detection (ex = 270 nm, em = 310 nm). the benefit of this direct injection of deproteinized plasma is to avoid the use of an internal standard. the detection limit was 0.1 pmol (4.0 nmol/l). the recovery of mda spiked in different human plasma samples was 95.3% (n = 25; r.s.d. 5.1%) for the hydrolysation procedure. the total and free mda in plasma of 15 healthy male volunteers are 426 +/- 29.8 nmol/l and 153 9.6 nmol/l, respectively. (c) 2006 elsevier b.v. all rights reserved. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; BOUND MALONDIALDEHYDE ; LIPID-PEROXIDATION ; MASS-SPECTROMETRY ; TISSUE-INJURY ; FREE-RADICALS ; HUMAN URINE ; 2,4-DINITROPHENYLHYDRAZINE ; SERUM ; HYDRAZINE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000235769100016 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379839 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liu, GQ |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mao, JJ,Zhang, HW,Luo, J,et al. New method for hplc separation and fluorescence detection of malonaldehyde in normal human plasma[J]. Journal of chromatography b-analytical technologies in the biomedical and life sciences,2006,832(1):103-108. |
| APA | Mao, JJ.,Zhang, HW.,Luo, J.,Li, L.,Zhao, R.,...&Liu, GQ.(2006).New method for hplc separation and fluorescence detection of malonaldehyde in normal human plasma.Journal of chromatography b-analytical technologies in the biomedical and life sciences,832(1),103-108. |
| MLA | Mao, JJ,et al."New method for hplc separation and fluorescence detection of malonaldehyde in normal human plasma".Journal of chromatography b-analytical technologies in the biomedical and life sciences 832.1(2006):103-108. |
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来源：中国科学院大学
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