Chinese-scorpion (buthus martensi karsch) toxin bmk alpha iv, a novel modulator of sodium channels: from genomic organization to functional analysis
文献类型：期刊论文
| 作者 | Chai, Zhi-Fang; Zhu, Mang-Mang; Bai, Zhan-Tao; Liu, Tong; Tan, Miao; Pang, Xue-Yan; Ji, Yong-Hua |
| 刊名 | Biochemical journal
 |
| 出版日期 | 2006-11-01 |
| 卷号 | 399页码:445-453 |
| 关键词 | Buthus martensi (chinese scorpion) alpha-like neurotoxin iv (bmk alpha iv) Cockroach neuronal membrane Rat brain ii sodium channel alpha-subunit (rnav1.2) Rat cerebrocortical synaptosome |
| ISSN号 | 0264-6021 |
| DOI | 10.1042/bj20060035 |
| 通讯作者 | Ji, yong-hua(yhji@server.shcnc.ac.cn) |
| 英文摘要 | In the present study, bmk alpha iv, a novel modulator of sodium channels, was cloned from venomous glands of the chinese scorpion (buthus martensi karsch) and expressed successfully in escherichia coli. the bmk alpha iv gene is composed of two exons separated by a 503 bp intron. the mature polypeptide contains 66 amino acids. bmk alpha iv has potent toxicity in mice and cockroaches. surface-plasmon-resonance analysis found that bmk aiv could bind to both rat cerebrocortical synaptosomes and cockroach neuronal membranes, and shared similar binding sites on sodium channels with classical aah ii (alpha-mammal neurotoxin from the scorpion androctonus australis hector), bmk as (beta-like neurotoxin), bmk it2 (the depressant insectselective neurotoxin) and bmk abt (transitional neurotoxin), but not with bmk i (alpha-like neurotoxin). two-electrode voltage clamp recordings on rnav1.2 channels expressed in xenopus laevis oocytes revealed that bmk aiv increased the peak amplitude and prolonged the inactivation phase of na+ currents. the structural and pharmacological properties compared with those of other scorpion a-toxins suggests that bmk aiv represents a novel subgroup or functional hybrid of a-toxins and might be an evolutionary intermediate neurotoxin for a-toxins. |
| WOS关键词 | GATED NA+ CHANNELS ; MOLECULAR-MECHANISMS ; RECEPTOR-SITES ; FOS EXPRESSION ; NEUROTOXIN ; RAT ; VENOM ; PURIFICATION ; SEQUENCES ; BINDING |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000241938300009 |
| 出版者 | PORTLAND PRESS LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379898 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Ji, Yong-Hua |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Physiol, Inst Biol Sci, Grad Sch, Shanghai 200031, Peoples R China 2.Shanghai Univ, Sch Life Sci, Shanghai 200444, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chai, Zhi-Fang,Zhu, Mang-Mang,Bai, Zhan-Tao,et al. Chinese-scorpion (buthus martensi karsch) toxin bmk alpha iv, a novel modulator of sodium channels: from genomic organization to functional analysis[J]. Biochemical journal,2006,399:445-453. |
| APA | Chai, Zhi-Fang.,Zhu, Mang-Mang.,Bai, Zhan-Tao.,Liu, Tong.,Tan, Miao.,...&Ji, Yong-Hua.(2006).Chinese-scorpion (buthus martensi karsch) toxin bmk alpha iv, a novel modulator of sodium channels: from genomic organization to functional analysis.Biochemical journal,399,445-453. |
| MLA | Chai, Zhi-Fang,et al."Chinese-scorpion (buthus martensi karsch) toxin bmk alpha iv, a novel modulator of sodium channels: from genomic organization to functional analysis".Biochemical journal 399(2006):445-453. |
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来源：中国科学院大学
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