Polyploid induction by heat shock-induced meiosis and mitosis inhibition in the dwarf surfclam, mulinia lateralis say
文献类型：期刊论文
| 作者 | Yang, HP; Guo, XM |
| 刊名 | Aquaculture
 |
| 出版日期 | 2006-03-10 |
| 卷号 | 252期号:2-4页码:171-182 |
| 关键词 | Triploid Tetraploid Heat shock Meiosis Mitosis Dwarf surfclam Mulinia lateralis |
| ISSN号 | 0044-8486 |
| DOI | 10.1016/j.aquaculture.2005.07.017 |
| 通讯作者 | Guo, xm(xguo@hsrl.rutgers.edu) |
| 英文摘要 | Heat shock is an effective and widely used method for polyploid induction in fish, but has not been well studied in molluscs. in this study, heat shock was tested for polyploid induction through meiosis or mitosis inhibition in the dwarf surfclam, mulinia lateralis say. to determine the most effective temperature, different heat shocks (32, 35 and 38 degrees c) were applied to newly fertilized eggs at 8-11 min post-fertilization (mpf) to inhibit the extrusion of polar body 1 (pb1), and ended when the second polar body began to form in control groups. ploidy of resultant larvae was determined by flow cytometry (fcm). both 35 degrees c and 3 8 degrees c were effective in inhibiting pb1, but 38 degrees c led to complete mortality of larvae. heat shock of 32 degrees c only accelerated embryonic development without inhibiting pb1, as all resultant larvae were diploid. high percentages of triploids (86.3-98.5%) were produced by the 35 degrees c treatment as detected at the juvenile stage. different durations (10, 15 and 20 min) of the 35 degrees c heat shock were used for pb1-inhibition to test effects on polyploid formation, which produced primarily triploids (10 min) and pentaploids (20 min), but virtually no tetraploids. to inhibit mitosis i (m1) and mitosis ii (m2) for tetraploid induction, a heat shock of 35 t was applied to fertilized eggs at 35-37 and 50-57 mpf for about 20 min, producing up to 82.8% and 44.4% tetraploid larvae, respectively. most tetraploid larvae were abnormal and did not develop to the d-stage, and no tetraploids were observed at the juvenile stage. this study shows that heat shock is a highly effective method for polyploid induction in the dwarf surfclam and possibly in other molluscs also. (c) 2005 elsevier b.v all rights reserved. |
| WOS关键词 | CRASSOSTREA-GIGAS THUNBERG ; CHLAMYS-FARRERI JONES ; POLAR BODY-I ; TETRAPLOID INDUCTION ; CYTOCHALASIN-B ; PACIFIC OYSTER ; FENNEROPENAEUS-CHINENSIS ; INDUCED TRIPLOIDY ; ZHIKONG SCALLOP ; FERTILIZED-EGGS |
| WOS研究方向 | Fisheries ; Marine & Freshwater Biology |
| WOS类目 | Fisheries ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000235784200009 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379905 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Guo, XM |
| 作者单位 | 1.Rutgers State Univ, Inst Marine Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, HP,Guo, XM. Polyploid induction by heat shock-induced meiosis and mitosis inhibition in the dwarf surfclam, mulinia lateralis say[J]. Aquaculture,2006,252(2-4):171-182. |
| APA | Yang, HP,&Guo, XM.(2006).Polyploid induction by heat shock-induced meiosis and mitosis inhibition in the dwarf surfclam, mulinia lateralis say.Aquaculture,252(2-4),171-182. |
| MLA | Yang, HP,et al."Polyploid induction by heat shock-induced meiosis and mitosis inhibition in the dwarf surfclam, mulinia lateralis say".Aquaculture 252.2-4(2006):171-182. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。