Sanj, an atp-dependent picolinate-coa ligase, catalyzes the conversion of picolinate to picolinate-coa during nikkomycin biosynthesis in streptomyces ansochromogenes
文献类型：期刊论文
| 作者 | Niu, GQ; Liu, G; Tian, YQ; Tan, HR |
| 刊名 | Metabolic engineering
 |
| 出版日期 | 2006-05-01 |
| 卷号 | 8期号:3页码:183-195 |
| 关键词 | Nikkomycin biosynthesis Streptomyces ansochromogenes Sanj Picolinate-coa ligase Picolinate Adenylate-forming enzymes |
| ISSN号 | 1096-7176 |
| DOI | 10.1016/j.ymben.2005.12.002 |
| 通讯作者 | Tan, hr() |
| 英文摘要 | Nikkomycins, a group of peptidyl nucleoside antibiotics, are competitive inhibitors of chitin synthase. the nikkornycin biosynthetic gene cluster has been cloned previously from streptomyces ansochromogenes. the cluster contains 25 complete orfs including sanj. the sanj gene was inactivated by the insertion of a kanamycin resistance gene and the resulting disruption mutants failed to produce nikkomycins. moreover, the nikkomycin production was restored by complementation with a single copy of sanj. the deduced product of sanj bears striking sequence similarity with enzymes belonging to the adenylate-forming superfamily. sanj was overexpressed as a his6-tagged fusion protein in escherichia coli and purified to apparent homogeneity by affinity chromatography. the purified sanj demonstrated adenylate ligase activity in the presence of picolinate or its analogs (benzoate, nicotinate, 4-methoxybenzoate, 4-hydroxybenzoate), atp and mg2+. sanj was also found to catalyze the conversion of picolinate, benzoate, nicotinate to their corresponding coa esters and 4-methoxybenzoate, 4-hydroxybenzoate to their respective amp derivatives in vitro. this was unambiguously shown by using hplc and electrospray ionization mass spectrometry (est-ms) or by comparing the reaction product with an authentic standard of benzoyl-coa. these results indicated that sanj encodes an atp-dependent picolinate-coa ligase which is essential for nikkomycin biosynthesis. (c) 2006 elsevier inc. all rights reserved. |
| WOS关键词 | NUCLEOSIDE ANTIBIOTIC NIKKOMYCIN ; MOLECULAR CHARACTERIZATION ; TENDAE TU901 ; PEPTIDE SYNTHETASES ; METABOLIC PRODUCTS ; TRANSCRIBED GENES ; CRYSTAL-STRUCTURE ; ESCHERICHIA-COLI ; CLONING ; MICROORGANISMS |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000237888400001 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379921 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Tan, HR |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Niu, GQ,Liu, G,Tian, YQ,et al. Sanj, an atp-dependent picolinate-coa ligase, catalyzes the conversion of picolinate to picolinate-coa during nikkomycin biosynthesis in streptomyces ansochromogenes[J]. Metabolic engineering,2006,8(3):183-195. |
| APA | Niu, GQ,Liu, G,Tian, YQ,&Tan, HR.(2006).Sanj, an atp-dependent picolinate-coa ligase, catalyzes the conversion of picolinate to picolinate-coa during nikkomycin biosynthesis in streptomyces ansochromogenes.Metabolic engineering,8(3),183-195. |
| MLA | Niu, GQ,et al."Sanj, an atp-dependent picolinate-coa ligase, catalyzes the conversion of picolinate to picolinate-coa during nikkomycin biosynthesis in streptomyces ansochromogenes".Metabolic engineering 8.3(2006):183-195. |
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来源：中国科学院大学
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