An open conformation of switch i revealed by sar1-gdp crystal structure at low mg2+
文献类型：期刊论文
| 作者 | Rao, Yijian; Bian, Chuanbing; Yuan, Cai; Li, Yongdong; Chen, Liqing; Ye, Xiaoming; Huang, Zixiang; Huang, Mingdong |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2006-09-29 |
| 卷号 | 348期号:3页码:908-915 |
| 关键词 | Sar1 Crystal structure Copii assembly Dimerization |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2006.07.148 |
| 通讯作者 | Huang, mingdong(mhuang@fjirsm.ac.cn) |
| 英文摘要 | Mg2+ is essential for guanosine triphosphatase activity and plays key roles in guanine nucleotide binding and preserving the structural integrity of gtp-binding proteins. to understand the structural basis for mg2+ function during the gdp/gtp exchange process, we determined the crystal structure of delta 9-sar1-gdp at low mg2+ concentration at 1.8 angstrom. two sarl-gdp molecules in the crystal form a dimer with mg2+ presenting only in molecule b but not in molecule a. the absence of mg2+ induces significant conformational changes in the switch i region in molecule a that shows similarities with those of ha-ras bound to sos. the current structure reveals an important regulatory role for mg2+. we suggest that guanine nucleotide exchange factor may utilize this feature to generate an open conformation for gdp/gtp exchange. furthermore, we propose a mechanism for copii assembly and disassembly in which dimerization of sar1 plays an important role. (c) 2006 elsevier inc. all rights reserved. |
| WOS关键词 | GUANINE-NUCLEOTIDE EXCHANGE ; ENDOPLASMIC-RETICULUM EXPORT ; GTP-BINDING PROTEIN ; TRANSPORT VESICLES ; ESCHERICHIA-COLI ; COAT PROTEINS ; COPII COAT ; ACTIVATION ; SITE ; RAS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000240166500018 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379959 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Huang, Mingdong |
| 作者单位 | 1.Chinese Acad Sci, Fujian Inst Res Struct Matter, State Key Lab Struct Chem, Fuzhou 350002, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Univ Alabama, Dept Chem, Lab Struct Biol, Grad Program Biotechnol, Huntsville, AL 35899 USA 4.Univ Alabama, Dept Chem, Lab Struct Biol, Grad Program Chem & Mat Sci, Huntsville, AL 35899 USA |
| 推荐引用方式 GB/T 7714 | Rao, Yijian,Bian, Chuanbing,Yuan, Cai,et al. An open conformation of switch i revealed by sar1-gdp crystal structure at low mg2+[J]. Biochemical and biophysical research communications,2006,348(3):908-915. |
| APA | Rao, Yijian.,Bian, Chuanbing.,Yuan, Cai.,Li, Yongdong.,Chen, Liqing.,...&Huang, Mingdong.(2006).An open conformation of switch i revealed by sar1-gdp crystal structure at low mg2+.Biochemical and biophysical research communications,348(3),908-915. |
| MLA | Rao, Yijian,et al."An open conformation of switch i revealed by sar1-gdp crystal structure at low mg2+".Biochemical and biophysical research communications 348.3(2006):908-915. |
入库方式： iSwitch采集
来源：中国科学院大学
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