Cloning and expression analysis of skn-type dehydrin gene from bean in response to heavy metals
文献类型：期刊论文
| 作者 | Zhang, YX; Li, JM; Yu, F; Cong, L; Wang, LY; Burkard, G; Chai, TY |
| 刊名 | Molecular biotechnology
 |
| 出版日期 | 2006-03-01 |
| 卷号 | 32期号:3页码:205-217 |
| 关键词 | Heavy metal Dehydrin Phaseolus vulgaris l. |
| ISSN号 | 1073-6085 |
| 通讯作者 | Chai, ty(tychai@gucas.ac.cn) |
| 英文摘要 | A heavy metal responsive gene pvsr3 (genbank accession number u54703) encoding an acid dehydrin was isolated from a mercuric chloride-treated bean (phaseolus vidgaris l.) leaf cdna library by differential screening using cdnas derived from treated and untreated plants. the pvsr3 cdna is 981-bp long and has a 606-bp open-reading frame with a 202-residue-deduced amino acid sequence. the pvsr3 sequence contains two conserved repeats of the characteristic lysine-rich k segment (ekkgimdkikeklpg) preceded by an 8-serine residue stretch, whereas the y segment (deygnp) conserved motif is absent. the deduced protein has a calculated molecular weight of 23 kda and an isoelectric point of 5.2. sequence similarity and comparative analysis showed that pvsr3 shares 70 and 73% similarity with the dehydrin of poplar and pepper, respectively. southern hybridizations indicated that pvsr3 was a low copy-number gene. northern blot analysis revealed that pvsr3 mrna was weakly detected in seedling leaves. however, the gene expression was strongly stimulated by heavy metals, such as mercury, cadmium, arsenic, and copper, whereas virus infection and salt had little effect oil it. in contrast, pvsr3 was not responsive to drought or abscisic acid (aba), and was downregulated by uv radiation. furthermore, pvsr3 was upregulated by the exogenous signaling molecules, including salicylic acid (sa) and hydrogen peroxide (h2o2). it is suggested that pvsr3 is extremely related to heavy metal stress, and might play an important role in metal detoxification and resistance to the damage caused by heavy metals. |
| WOS关键词 | TRANSGENIC TOBACCO ; SACCHAROMYCES-CEREVISIAE ; FREEZING TOLERANCE ; CITRUS DEHYDRIN ; OSMOTIC-STRESS ; SALICYLIC-ACID ; ARABIDOPSIS ; PROTEIN ; PLANTS ; PHOSPHORYLATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000236774900003 |
| 出版者 | HUMANA PRESS INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2379979 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chai, TY |
| 作者单位 | 1.Grad Univ Chinese Acad Sci, Dept Biol, Beijing 100049, Peoples R China 2.China Univ Min & Technol, Sch Chem & Environm Engn, Dept Biol Engn, Beijing 100083, Peoples R China 3.Univ Strasbourg 1, CNRS, Inst Biol Mol Plantes, F-67084 Strasbourg, France |
| 推荐引用方式 GB/T 7714 | Zhang, YX,Li, JM,Yu, F,et al. Cloning and expression analysis of skn-type dehydrin gene from bean in response to heavy metals[J]. Molecular biotechnology,2006,32(3):205-217. |
| APA | Zhang, YX.,Li, JM.,Yu, F.,Cong, L.,Wang, LY.,...&Chai, TY.(2006).Cloning and expression analysis of skn-type dehydrin gene from bean in response to heavy metals.Molecular biotechnology,32(3),205-217. |
| MLA | Zhang, YX,et al."Cloning and expression analysis of skn-type dehydrin gene from bean in response to heavy metals".Molecular biotechnology 32.3(2006):205-217. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。