Involvement of sphingoid bases in mediating reactive oxygen intermediate production and programmed cell death in arabidopsis
文献类型：期刊论文
| 作者 | Shi, Lihua1,2; Bielawski, Jacek3; Mu, Jinye1,2; Dong, Haili1,2; Teng, Chong1,2; Zhang, Jian1; Yang, Xiaohui1; Tomishige, Nario4; Hanada, Kentaro4; Hannun, Yusuf A.3 |
| 刊名 | Cell research
 |
| 出版日期 | 2007-12-01 |
| 卷号 | 17期号:12页码:1030-1040 |
| 关键词 | Arabidopsis Fumonisin b1 resistant11-1 Pcd Rois Sphingolipids |
| ISSN号 | 1001-0602 |
| DOI | 10.1038/cr.2007.100 |
| 通讯作者 | Zuo, jianru(jrzuo@genetics.ac.cn) |
| 英文摘要 | Sphingolipids have been suggested to act as second messengers for an array of cellular signaling activities in plant cells, including stress responses and programmed cell death (pcd). however, the mechanisms underpinning these processes are not well understood. here, we report that an arabidopsis mutant, fumonisin b resistant11-1 (fbr11-1), which fails to generate reactive oxygen inter-mediates (rois), is incapable of initiating pcd when the mutant is challenged by famonisin b-1(fb1), a specific inhibitor of ceramide synthase. molecular analysis indicated that fbr11 encodes a long-chain base 1 (lcb1) subunit of serine palmitoyltransferase (spt), which catalyzes the first rate-limiting step of de novo sphingolipid synthesis. mass spectrometric analysis of the sphingolipid concentrations revealed that whereas the fbr11-1 mutation did not affect basal levels of sphingoid bases, the mutant showed attenuated formation of sphingoid bases in response to fb1. by a direct feeding experiment, we show that the free sphingoid bases dilrydrosphingosine, phytosphingosine and sphingosine efficiently induce roi generation followed by cell death. conversely, roi generation and cell death induced by dilrydrosphingosine were specifically blocked by its phosphorylated form dihydrosphingosine-1-phosphate in a dose-dependent manner, suggesting that the maintenance of homeostasis between a free sphingoid base and its phosphorylated derivative is critical to determining the cell fate. because alterations of the sphingolipid level occur prior to the roi production, we propose that the free sphingoid bases are involved in the control of pcd in arabidopsis, presumably through the regulation of the roi level upon receiving different developmental or environmental cues. |
| WOS关键词 | PLANT-DISEASE RESISTANCE ; F-SP LYCOPERSICI ; SERINE PALMITOYLTRANSFERASE ; HYPERSENSITIVE RESPONSE ; AAL-TOXIN ; SIGNALING PATHWAYS ; MAMMALIAN-CELLS ; LCB1 SUBUNIT ; GENE ; SPHINGOLIPIDS |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000252607800009 |
| 出版者 | INST BIOCHEMISTRY & CELL BIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380006 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zuo, Jianru |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Med Univ S Carolina, Dept Biochem & Mol Biol, Charleston, SC 29425 USA 4.Natl Inst Infect Dis, Dept Biochem & Cell Biol, Shinjuku Ku, Tokyo 1628640, Japan |
| 推荐引用方式 GB/T 7714 | Shi, Lihua,Bielawski, Jacek,Mu, Jinye,et al. Involvement of sphingoid bases in mediating reactive oxygen intermediate production and programmed cell death in arabidopsis[J]. Cell research,2007,17(12):1030-1040. |
| APA | Shi, Lihua.,Bielawski, Jacek.,Mu, Jinye.,Dong, Haili.,Teng, Chong.,...&Zuo, Jianru.(2007).Involvement of sphingoid bases in mediating reactive oxygen intermediate production and programmed cell death in arabidopsis.Cell research,17(12),1030-1040. |
| MLA | Shi, Lihua,et al."Involvement of sphingoid bases in mediating reactive oxygen intermediate production and programmed cell death in arabidopsis".Cell research 17.12(2007):1030-1040. |
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来源：中国科学院大学
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