Selective enrichment of endogenous peptides by chemically modified porous nanoparticles for peptidome analysis
文献类型：期刊论文
| 作者 | Tian, Ruijun2; Ren, Lianbing1; Ma, Huaijun3; Li, Xin2; Hu, Lianghai2; Ye, Mingliang2; Wu, Ren'an2; Tian, Zhijian3; Liu, Zhen1; Zou, Hanfa2 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2009-02-20 |
| 卷号 | 1216期号:8页码:1270-1278 |
| 关键词 | Peptidomics Ordered porous nanoparticles Strong ion exchange Reversed phase Two-dimensional separation Mouse liver |
| ISSN号 | 0021-9673 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | we report the development of a combined strategy for high capacity, comprehensive enrichment of endogenous peptide from complex biological samples at natural ph condition. mcm-41 nanoparticles with highly ordered nanoscale pores (i.e. 4.8 nm) and high-surface area (i.e. 751 m(2)/g) were synthesized and modified with strong cation-exchange (scx-mcm-41) and strong anion-exchange (sax-mcm-41) groups. the modified nanoparticles demonstrated good size-exclusion effect for the adsorption of standard protein lysozyme with molecular weight (mw) of ca. 15 kda; and the peptides with mw lower than this value can be well adsorbed. step elution of the enriched peptides with five salt concentrations presented that both modified nanoparticles have high capacity and complementarity for peptides enrichment, and the sax-mcm-41 nanoparticles has obviously high selectivity for acidic peptides with pi (isoelectric point) lower than 4. large-scale enrichment of endogenous peptides in 2 mg mouse liver extract was achieved by further combination of scx-mcm-41 and sax-mcm-41 with unmodified mcm-41 nanoparticles. on-line 2d nano-lc/ms/ms was applied to analyze the enriched samples, and 2721 unique peptides were identified in total. two-dimensional analysis of mw versus pi distribution combined with abundance of the identified peptides demonstrated that the three types of nanoparticles have comprehensive complementarity for peptidome enrichment. (c) 2008 published by elsevier b.v. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | mass-spectrometry ; biomarker discovery ; mesoporous silica ; human plasma ; functionalized mcm-41 ; membrane-proteins ; proteome ; serum ; identification ; chromatography |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000263740300008 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/101509]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nanjing Univ, Sch Chem & Chem Engn, Nanjing 210093, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116013, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Appl Catalysis, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian, Ruijun,Ren, Lianbing,Ma, Huaijun,et al. Selective enrichment of endogenous peptides by chemically modified porous nanoparticles for peptidome analysis[J]. journal of chromatography a,2009,1216(8):1270-1278. |
| APA | Tian, Ruijun.,Ren, Lianbing.,Ma, Huaijun.,Li, Xin.,Hu, Lianghai.,...&Zou, Hanfa.(2009).Selective enrichment of endogenous peptides by chemically modified porous nanoparticles for peptidome analysis.journal of chromatography a,1216(8),1270-1278. |
| MLA | Tian, Ruijun,et al."Selective enrichment of endogenous peptides by chemically modified porous nanoparticles for peptidome analysis".journal of chromatography a 1216.8(2009):1270-1278. |
入库方式： OAI收割
来源：大连化学物理研究所
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