Glycoproteomic Reactor for Human Plasma
文献类型：期刊论文
| 作者 | Zhou, Hu; Hou, Weimin2; Denis, Nicholas J.; Zhou, Houjiang3; Vasilescu, Julian; Zou, Hanfa3; Figeys, Daniel1,2 |
| 刊名 | journal of proteome research
 |
| 出版日期 | 2009-02-01 |
| 卷号 | 8期号:2页码:556-566 |
| 关键词 | glycoproteins mass spectrometry proteomics plasma proteomic reactor microscale |
| ISSN号 | 1535-3893 |
| 产权排序 | 3;4 |
| 通讯作者 | 邹汉法 ; daniel figeys |
| 英文摘要 | we describe the development of a glycoproteomic reactor that combines multiple biochemical and chemical protein processing into a single device for the study of n-glycosylated proteins. the glycoproteins are first enriched by concanavalin a affinity chromatography and then transferred onto and efficiently processed in the glycoproteomic reactor. this glycoproteomic reactor combines protein concentration and purification, disulfide bond reduction, peptide-n-glycosidase-mediated (18)o-labeling and deglycosylation, alkylation, tryptic digestion and ph based fractionation in a device that has an interstitial volume (reaction volume) of similar to 1 mu l. we demonstrated the potential of the glycoproteomic reactor using human plasma. under stringent criteria, 82 unique glycopeptides representing 41 unique glycoproteins were identified from as little as 5 mu l of human plasma. our glycoproteomic reactor reduces the sample processing time to less than 1.5 h, reduces the reagent consumption while providing over 1000-fold concentration of the sample, provides efficient removal of high concentration of glycan buffer, and, finally, allows both glycopeptides and nonglycosylated tryptic peptides to be analyzed by the mass spectrometer which provides much greater protein coverage and more reliable identifications. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | n-linked glycoproteins ; multilectin affinity-chromatography ; mass-spectrometry ; proteomic reactor ; hydrazide chemistry ; glycosylation sites ; serum-proteins ; identification ; capture ; cancer |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000263193300016 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/101515]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Univ Ottawa, Ottawa Inst Syst Biol, Dept Biochem Microbiol & Immunol, Fac Med, Ottawa, ON K1H 8M5, Canada 2.Univ Ottawa, Fac Sci, Dept Chem, Ottawa, ON K1N 6N5, Canada 3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Hu,Hou, Weimin,Denis, Nicholas J.,et al. Glycoproteomic Reactor for Human Plasma[J]. journal of proteome research,2009,8(2):556-566. |
| APA | Zhou, Hu.,Hou, Weimin.,Denis, Nicholas J..,Zhou, Houjiang.,Vasilescu, Julian.,...&Figeys, Daniel.(2009).Glycoproteomic Reactor for Human Plasma.journal of proteome research,8(2),556-566. |
| MLA | Zhou, Hu,et al."Glycoproteomic Reactor for Human Plasma".journal of proteome research 8.2(2009):556-566. |
入库方式： OAI收割
来源：大连化学物理研究所
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