Mutagenesis of ser(24) of cytochrome b(559) alpha subunit affects psii activities in chlamydomonas reinhardtii
文献类型：期刊论文
| 作者 | Ma JingJing; Li LiangBi; Jing YuXiang; Kuang TingYun |
| 刊名 | Chinese science bulletin
 |
| 出版日期 | 2007-04-01 |
| 卷号 | 52期号:7页码:896-902 |
| 关键词 | Chlamydomonas reinhardtii Chloroplast transformation Cytochrome b(559) Site-directed mutagenesis Photosystem two |
| ISSN号 | 1001-6538 |
| DOI | 10.1007/s11434-007-0147-7 |
| 通讯作者 | Kuang tingyun(kuangty@ibcas.ac.cn) |
| 英文摘要 | In order to study the functions of cytochrome b(559) (cyt b(559)) in photosystem two (psii) activity, mutant s24f of chlamydomonas reinhardtii was constructed using site directed mutagenesis, in which serine (24) (ser(24)) locating downstream of histidine(23) (his(23)) in a subunit of cyt b(559) was replaced by phenylalanine (phe). physiological and biochemical analysis showed that mutant s24f could be grown photoautotrophically or photoheterotrophically. however, their growth rate was slower either on hsm or tap medium than that of the control; analysis of psii activity revealed that its oxygen evolution was about 71% of wild type (wt); the photochemical efficiency of psii (fvifm) of s24f was reduced 0.23 compared with wt, s24f was more sensitive to strong light irradiance than the wild type; furthermore, sds-page and western-blotting analysis indicated that the expression levels of a subunit of cyt b(559), lhcii and psbo of s24f were a little less than those of the wild type. overall, these data suggests that ser24 plays a significant role in making cyt b(559) structure maintain psii complex activity of oxygen evolution although it is not directly bound to heme group. |
| WOS关键词 | PHOTOSYNTHETIC ELECTRON-TRANSPORT ; SITE-DIRECTED MUTAGENESIS ; PHOTOSYSTEM-II ; B-559 ; LIGHT ; PHOTOINHIBITION ; CHLOROPLASTS ; OXIDATION ; MUTANTS ; PROTEIN |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000246677500005 |
| 出版者 | SCIENCE CHINA PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380087 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Kuang TingYun |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma JingJing,Li LiangBi,Jing YuXiang,et al. Mutagenesis of ser(24) of cytochrome b(559) alpha subunit affects psii activities in chlamydomonas reinhardtii[J]. Chinese science bulletin,2007,52(7):896-902. |
| APA | Ma JingJing,Li LiangBi,Jing YuXiang,&Kuang TingYun.(2007).Mutagenesis of ser(24) of cytochrome b(559) alpha subunit affects psii activities in chlamydomonas reinhardtii.Chinese science bulletin,52(7),896-902. |
| MLA | Ma JingJing,et al."Mutagenesis of ser(24) of cytochrome b(559) alpha subunit affects psii activities in chlamydomonas reinhardtii".Chinese science bulletin 52.7(2007):896-902. |
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来源：中国科学院大学
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