Eisenia fetida protease-iii-1 functions in both fibrinolysis and fibrogenesis
文献类型：期刊论文
| 作者 | Zhao, Jing; Pan, Rong; He, Jian; Liu, Ying; Li, Dong-Feng; He, Rong-Qiao |
| 刊名 | Journal of biomedicine and biotechnology
 |
| 出版日期 | 2007 |
| 页码 | 10 |
| ISSN号 | 1110-7243 |
| DOI | 10.1155/2007/97654 |
| 通讯作者 | Zhao, jing() |
| 英文摘要 | The fibrinolytic function of earthworm protease-iii-1 (efp-iii-1) has been studied in recent years. here, we found that efp-iii-1 acted not only in fibrinogenolysis, but also in fibrogenesis. we have used efp-iii-1 to hydrolyze fibrinogen, and to activate plasminogen and prothrombin. based on the n-terminal sequences of the hydrolytic fragments, efp-iii-1 was showed to specifically recognize the carboxylic sites of arginine and lysine. analyses by fibrinogenolysis mapping and amino acid sequencing revealed that the isozyme could cleave the alpha, beta, and gamma chains of fibrinogen, showing a high alpha-fibrinogenase, moderate beta-fibrinogenase, and low gamma-fibrinogenase activities. interestingly, efp-iii-1 activated plasminogen and released active plasmin, suggesting a tpa-like function. furthermore, efp-iii-1 showed a factor xa-like function on prothrombin, producing alpha-thrombin. the function in both activating prothrombin and catalyzing fibrinogenolysis suggests that efp-iii-1 may play a role in the balance between procoagulation and anticoagulation. copyright (c) 2007 jing zhao et al. this is an open access article distributed under the creative commons attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| WOS关键词 | EARTHWORM LUMBRICUS-RUBELLUS ; AMINO-ACID-SEQUENCE ; PLASMINOGEN ACTIVATION ; INTESTINAL-ABSORPTION ; ENZYME III-1 ; ALPHA-CHAIN ; PURIFICATION ; FIBRINOGEN ; LUMBROKINASE ; AUTOLYSATE |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Research & Experimental Medicine |
| WOS类目 | Biotechnology & Applied Microbiology ; Medicine, Research & Experimental |
| 语种 | 英语 |
| 出版者 | HINDAWI PUBLISHING CORPORATION |
| WOS记录号 | WOS:000247594700001 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380091 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhao, Jing |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, Baiao Pharmaceut Beijing CL, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.S China Normal Univ, Coll Life Sci, Guangzhou 510631, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Jing,Pan, Rong,He, Jian,et al. Eisenia fetida protease-iii-1 functions in both fibrinolysis and fibrogenesis[J]. Journal of biomedicine and biotechnology,2007:10. |
| APA | Zhao, Jing,Pan, Rong,He, Jian,Liu, Ying,Li, Dong-Feng,&He, Rong-Qiao.(2007).Eisenia fetida protease-iii-1 functions in both fibrinolysis and fibrogenesis.Journal of biomedicine and biotechnology,10. |
| MLA | Zhao, Jing,et al."Eisenia fetida protease-iii-1 functions in both fibrinolysis and fibrogenesis".Journal of biomedicine and biotechnology (2007):10. |
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来源：中国科学院大学
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