An improved primer extension method for detection of mrna start-points using non-radioactive digoxigenin-labeling primers
文献类型：期刊论文
| 作者 | Qi, Xiaoting; Chai, Xiaoqing; Chai, Tuanyao |
| 刊名 | Biotechnology letters
 |
| 出版日期 | 2007-07-01 |
| 卷号 | 29期号:7页码:1125-1128 |
| 关键词 | Digoxigenin Digoxigenin-labeling and detection system Primer extension Transcriptional start site |
| ISSN号 | 0141-5492 |
| DOI | 10.1007/s10529-007-9356-z |
| 通讯作者 | Qi, xiaoting(qixiaoting@mail.cnu.edu.cn) |
| 英文摘要 | An improved primer extension method using non-radioactive digoxigenin (dig)-labeling primers is described which uses a commercially-available dig-labeling and detection system to perform alternative hybridization-based "labeling" procedure of dna markers and dig chemiluminescent detection assay. the time-consuming annealing step and relatively low specificity of conventional protocol are also improved considerably by an application of one-step primer/mrna annealing procedure and subsequent high-temperature reverse transcription reaction. this new protocol is convenient, simple, cost-effective and safe, and can allow the detection of even low abundance mrna start-points in enriched poly(a)(+) rna samples. |
| WOS关键词 | MOSAIC-VIRUS DNA ; HEAVY-METAL ; TRANSCRIPTION ; SEQUENCES |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000246747900021 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380139 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Qi, Xiaoting |
| 作者单位 | 1.Capital Normal Univ, Coll Life Sci, Beijing 100037, Peoples R China 2.Grad Univ Chinese Acad Sci, Dept Biol, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Xiaoting,Chai, Xiaoqing,Chai, Tuanyao. An improved primer extension method for detection of mrna start-points using non-radioactive digoxigenin-labeling primers[J]. Biotechnology letters,2007,29(7):1125-1128. |
| APA | Qi, Xiaoting,Chai, Xiaoqing,&Chai, Tuanyao.(2007).An improved primer extension method for detection of mrna start-points using non-radioactive digoxigenin-labeling primers.Biotechnology letters,29(7),1125-1128. |
| MLA | Qi, Xiaoting,et al."An improved primer extension method for detection of mrna start-points using non-radioactive digoxigenin-labeling primers".Biotechnology letters 29.7(2007):1125-1128. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。