Molecular evolution of paclitaxel biosynthetic genes TS and DBAT of Taxus species
文献类型：期刊论文
| 作者 | Hao, Da Cheng; Yang, Ling; Huang, Beili |
| 刊名 | genetica
 |
| 出版日期 | 2009-03-01 |
| 卷号 | 135期号:2页码:123-135 |
| 关键词 | Adaptive evolution 10-Deacetylbaccatin III-10 beta-O-acetyltransferase Paclitaxel Positive selection Taxadiene synthase Taxus |
| 产权排序 | 1;1 |
| 通讯作者 | 杨凌 |
| 英文摘要 | evolutionary patterns of sequence divergence were analyzed in genes from the conifer genus taxus (yew), encoding paclitaxel biosynthetic enzymes taxadiene synthase (ts) and 10-deacetylbaccatin iii-10 beta-o-acetyltransferase (dbat). n-terminal fragments of ts, full-length dbat and internal transcribed spacer (its) were amplified from 15 closely related taxus species and sequenced. premature stop codons were not found in ts and dbat sequences. codon usage bias was not found, suggesting that synonymous mutations are selectively neutral. ts and dbat gene trees are not consistent with the its tree, where species formed monophyletic clades. in fact, for both genes, alleles were sometimes shared across species and parallel amino acid substitutions were identified. while both ts and dbat are, overall, under purifying selection, we identified a number of amino acids of ts under positive selection based on inference using maximum likelihood models. positively selected amino acids in the n-terminal region of ts suggest that this region might be more important for enzyme function than previously thought. moreover, we identify lineages with significantly elevated rates of amino acid substitution using a genetic algorithm. these findings demonstrate that the pattern of adaptive paclitaxel biosynthetic enzyme evolution can be documented between closely related taxus species, where species-specific taxane metabolism has evolved recently. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | genetics & heredity |
| 研究领域[WOS] | genetics & heredity |
| 关键词[WOS] | amino-acid sites ; taxol biosynthesis ; taxadiene synthase ; selection pressure ; escherichia-coli ; committed step ; nucleotide ; models ; substitutions ; cyclization |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000263058600001 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/101531]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hao, Da Cheng,Yang, Ling,Huang, Beili. Molecular evolution of paclitaxel biosynthetic genes TS and DBAT of Taxus species[J]. genetica,2009,135(2):123-135. |
| APA | Hao, Da Cheng,Yang, Ling,&Huang, Beili.(2009).Molecular evolution of paclitaxel biosynthetic genes TS and DBAT of Taxus species.genetica,135(2),123-135. |
| MLA | Hao, Da Cheng,et al."Molecular evolution of paclitaxel biosynthetic genes TS and DBAT of Taxus species".genetica 135.2(2009):123-135. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。