Palladin regulates cell and extracellular matrix interaction through maintaining normal actin cytoskeleton architecture and stabilizing beta1-integrin
文献类型：期刊论文
| 作者 | Liu, Xue-Song; Luo, Hui-Jun; Yang, Hua; Wang, Long; Kong, Hui; Jin, Yue-E; Wang, Fang; Gu, Ming-Min; Chen, Zhu; Lu, Zhen-Yu |
| 刊名 | Journal of cellular biochemistry
 |
| 出版日期 | 2007-04-01 |
| 卷号 | 100期号:5页码:1288-1300 |
| 关键词 | Palladin Cell adhesion Cell spreading Rho gtpase Beta 1-integrin |
| ISSN号 | 0730-2312 |
| DOI | 10.1002/jcb.21126 |
| 通讯作者 | Wang, zhu-gang(zhugangw@shsmu.edu.cn) |
| 英文摘要 | Cell and extracellular matrix (ecm) interaction plays an important role in development and normal cellular function. cell adhesion and cell spreading on ecm are two basic cellular behaviors related to cell-ecm interaction. here we show that palladin, a novel actin cytoskeleton-associated protein, is actively involved in the regulation of cell-ecm inte'raction. it was found that palladin-deficient mouse embryonic fibroblasts (mefs) display decreased cell adhesion and compromised cell spreading on various ecms. disorganized actin cytoskeleton architecture characterized by faint stress fibers, less lamellipodia and focal adhesions can account for the weakened cell-ecm interaction in palladin(-/-) mefs. furthermore, decreased polymerized filament actin and increased globular actin can be observed in palladin(-/-) mefs, strongly suggesting that palladin is essential for the formation or stabilization of polymerized filament actin. elevated phospho-cofilin level and proper responses in cofilin phosphorylation to either rho signal agonist or antagonist in palladin(-/-) mefs indicate that disrupted stress fibers in palladin(-/-) mefs is not associated with cofilin phosphorylation. more interestingly, the protein level of ecm receptor beta 1-integrin is dramatically decreased in mefs lacking palladin. down-regulation of beta 1-integrin protein can be restored by proteasome inhibitor mg-132 treatment. all these data implicate that palladin is essential for cell-ecm interaction through maintaining normal actin cytoskeleton architecture and stabilizing beta 1-integrin protein. |
| WOS关键词 | ALPHA-ACTININ ; POTENTIAL TARGET ; ARP2/3 COMPLEX ; FOCAL ADHESION ; STRESS FIBERS ; RHO-GTPASES ; F-ACTIN ; INTEGRIN ; PROTEIN ; PATHWAY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000245154200020 |
| 出版者 | WILEY-LISS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380158 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Zhu-Gang |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Lab Genet Engn, Dept Med Genet, Inst Hlth Sci,Med Sch, Shanghai 200025, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai 200025, Peoples R China 3.Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, State Key Lab Med Genom, Shanghai 200025, Peoples R China 4.Shanghai Res Ctr Model Organisms, Shanghai 201203, Peoples R China 5.Chinese Acad Sci, Grad Sch, Shanghai 200025, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xue-Song,Luo, Hui-Jun,Yang, Hua,et al. Palladin regulates cell and extracellular matrix interaction through maintaining normal actin cytoskeleton architecture and stabilizing beta1-integrin[J]. Journal of cellular biochemistry,2007,100(5):1288-1300. |
| APA | Liu, Xue-Song.,Luo, Hui-Jun.,Yang, Hua.,Wang, Long.,Kong, Hui.,...&Wang, Zhu-Gang.(2007).Palladin regulates cell and extracellular matrix interaction through maintaining normal actin cytoskeleton architecture and stabilizing beta1-integrin.Journal of cellular biochemistry,100(5),1288-1300. |
| MLA | Liu, Xue-Song,et al."Palladin regulates cell and extracellular matrix interaction through maintaining normal actin cytoskeleton architecture and stabilizing beta1-integrin".Journal of cellular biochemistry 100.5(2007):1288-1300. |
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来源：中国科学院大学
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