Rna interference-mediated silencing of phytochelatin synthase gene reduce cadmium accumulation in rice seeds
文献类型：期刊论文
| 作者 | Li, Jiang-Chuan; Guo, Jiang-Bo; Xu, Wen-Zhong; Ma, Mi |
| 刊名 | Journal of integrative plant biology
 |
| 出版日期 | 2007-07-01 |
| 卷号 | 49期号:7页码:1032-1037 |
| 关键词 | Accumulation Cadmium Phytochelatin synthase Rice Rna interference |
| ISSN号 | 1672-9072 |
| DOI | 10.1111/j.1672-9072.2007.00473.x |
| 通讯作者 | Ma, mi(mami@ibcas.ac.cn) |
| 英文摘要 | Phytochelatins (pcs) play an important role in heavy metal resistance and accumulation. to reduce the accumulation of cadmium (cd) in rice seeds, the expression of phytochelatin synthase (pcs) gene ospcs1 was suppressed by rna interference (rnai). a hairpin construct of a pcs fragment was designed in the prnai-ospcs1 under the control of zmm1, a seed-specific promoter from maize. the construct was introduced into rice (japonica) through agrobacterium tumefaciens. the rnai rice plantlets were selected and cultivated in pots exposured to 10 mg/kg cd. the transcriptional level of ospcs1 declined in seeds of some rnai rice compared to the wild type. as a result cd accumulation was reduced by about half in the seeds of rnai rice. as expected, no apparent difference of growth appeared between rnai and wild-type plants. the results suggest that this new approach can be used to control heavy metal accumulation in crops. |
| WOS关键词 | HEAVY-METAL DETOXIFICATION ; ARABIDOPSIS-THALIANA ; BINDING PEPTIDES ; PLANTS ; TOLERANCE ; METALLOTHIONEINS ; BIOSYNTHESIS ; ROLES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS类目 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000247442100008 |
| 出版者 | WILEY-BLACKWELL |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380180 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Ma, Mi |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jiang-Chuan,Guo, Jiang-Bo,Xu, Wen-Zhong,et al. Rna interference-mediated silencing of phytochelatin synthase gene reduce cadmium accumulation in rice seeds[J]. Journal of integrative plant biology,2007,49(7):1032-1037. |
| APA | Li, Jiang-Chuan,Guo, Jiang-Bo,Xu, Wen-Zhong,&Ma, Mi.(2007).Rna interference-mediated silencing of phytochelatin synthase gene reduce cadmium accumulation in rice seeds.Journal of integrative plant biology,49(7),1032-1037. |
| MLA | Li, Jiang-Chuan,et al."Rna interference-mediated silencing of phytochelatin synthase gene reduce cadmium accumulation in rice seeds".Journal of integrative plant biology 49.7(2007):1032-1037. |
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来源：中国科学院大学
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