Genetic analysis of a novel plasmid pzmx101 from halorubrum saccharovorum: determination of the minimal replicon and comparison with the related haloarchaeal plasmid pscm201
文献类型：期刊论文
| 作者 | Zhou, Ligang; Zhou, Meixian; Sun, Chaomin; Xiang, Hua; Tan, Huarong |
| 刊名 | Fems microbiology letters
 |
| 出版日期 | 2007-05-01 |
| 卷号 | 270期号:1页码:104-108 |
| 关键词 | Haloarchaea Pzmx101 Minimal replicon Shuttle vector |
| ISSN号 | 0378-1097 |
| DOI | 10.1111/j.1574-6968.2007.00656.x |
| 通讯作者 | Xiang, hua(xiangh@sun.im.ac.cn) |
| 英文摘要 | The dna sequence of a novel haloarchaeal plasmid pzmx101 (3918 bp) from halorubrum saccharovorum was determined and six orfs were predicted. the largest orf encodes a putative replication initiation protein repa, which shares 40% sequence similarity with the rep201 of a theta-replication plasmid pscm201 recently isolated from haloarcula, suggesting that pzmx101 might replicate via a theta-type mechanism. using pzmx101 as the only haloarchaeal replicon, a shuttle vector pzmx108 was constructed and successfully transformed into haloferax volcanii ds70. based on this in vivo system, the minimal replicon (1978 bp) of pzmx101 was determined. it is composed of the repa gene plus c. 400-bp upstream and 300-bp downstream sequences. significantly, the putative replication origin of pzmx101 and that of pscm201 contain different types of sequence motifs, and these two plasmids exhibit distinct host preference for haloferax and haloarcula, respectively. |
| WOS关键词 | MOLECULAR CHARACTERIZATION ; HALOALKALIPHILIC ARCHAEON ; NUCLEOTIDE-SEQUENCE ; HALOBACTERIUM ; REPLICATION ; TRANSFORMATION ; VECTORS ; GRB |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000245674800014 |
| 出版者 | BLACKWELL PUBLISHING |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380196 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Xiang, Hua |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100080, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Ligang,Zhou, Meixian,Sun, Chaomin,et al. Genetic analysis of a novel plasmid pzmx101 from halorubrum saccharovorum: determination of the minimal replicon and comparison with the related haloarchaeal plasmid pscm201[J]. Fems microbiology letters,2007,270(1):104-108. |
| APA | Zhou, Ligang,Zhou, Meixian,Sun, Chaomin,Xiang, Hua,&Tan, Huarong.(2007).Genetic analysis of a novel plasmid pzmx101 from halorubrum saccharovorum: determination of the minimal replicon and comparison with the related haloarchaeal plasmid pscm201.Fems microbiology letters,270(1),104-108. |
| MLA | Zhou, Ligang,et al."Genetic analysis of a novel plasmid pzmx101 from halorubrum saccharovorum: determination of the minimal replicon and comparison with the related haloarchaeal plasmid pscm201".Fems microbiology letters 270.1(2007):104-108. |
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来源：中国科学院大学
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