Development of a heat shock inducible and inheritable rnai system in silkworm
文献类型：期刊论文
| 作者 | Dai, Hongjiu1,2,4; Jiang, Rongjing1,2; Wang, Jue2; Xu, Guojiang2; Cao, Meixun1; Wang, Zhugang2; Fei, Jian2,3 |
| 刊名 | Biomolecular engineering
 |
| 出版日期 | 2007-12-01 |
| 卷号 | 24期号:6页码:625-630 |
| 关键词 | Transgenic silkworm Rnai Heat shock Eclosion hormone Bombyx mori |
| ISSN号 | 1389-0344 |
| DOI | 10.1016/j.bioeng.2007.10.004 |
| 通讯作者 | Jiang, rongjing(rjjiang@sibs.ac.cn) |
| 英文摘要 | A heat shock inducible and inheritable rna interference (rnai) system was developed in the silkworm (bombyx mori). rnai transgenic silkworms were generated by injecting silkworm eggs with a piggybac transposon plasmid carrying rnai sequence against target gene driven by the drosophila heat shock protein 70 (hsp70) promoter and the helper plasmid expressing piggybac transposase. the transgenic egfp gene and the endogenous eclosion hormone (eh) gene were chosen respectively as the target genes. in the rnai transgenic, silkworms, heat shock at 42 degrees c significantly and specifically reduced the expression of egfp or eh gene in silkworms according to the corresponding rnai targeting sequence but not in silkworms with the irrelevant rnai sequence demonstrating the efficiency and specificity of the rnai effect. heat shock in the pupal stage hampered pupal-adult eclosion and reduced egg fertility in eh rnai transgenic silkworms but not in the wild type or egfp rnai transgenic silkworms. the establishment of this heat inducible and inheritable conditional rna interference system in silkworms provided an approach for the first time to dissect the functions of target genes in silkworms at different stages. (c) 2007 elsevier b.v. all rights reserved. |
| WOS关键词 | DOUBLE-STRANDED-RNA ; MANDUCA-SEXTA ; ECLOSION HORMONE ; TOBACCO HORNWORM ; BOMBYX-MORI ; GENE ; EXPRESSION ; SEQUENCE ; ECDYSIS ; INTERFERENCE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS类目 | Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000252063800007 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380248 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Jiang, Rongjing |
| 作者单位 | 1.Chinese Acad Sci, SIBS, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 2.Shanghai Nan Fang Res Ctr Model Organism, Shanghai 201203, Peoples R China 3.Tongji Univ, Shanghai 200092, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dai, Hongjiu,Jiang, Rongjing,Wang, Jue,et al. Development of a heat shock inducible and inheritable rnai system in silkworm[J]. Biomolecular engineering,2007,24(6):625-630. |
| APA | Dai, Hongjiu.,Jiang, Rongjing.,Wang, Jue.,Xu, Guojiang.,Cao, Meixun.,...&Fei, Jian.(2007).Development of a heat shock inducible and inheritable rnai system in silkworm.Biomolecular engineering,24(6),625-630. |
| MLA | Dai, Hongjiu,et al."Development of a heat shock inducible and inheritable rnai system in silkworm".Biomolecular engineering 24.6(2007):625-630. |
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来源：中国科学院大学
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