Study of multiple binding constants of dexamethasone with human serum albumin by capillary electrophoresis-frontal analysis and multivariate regression
文献类型：期刊论文
| 作者 | Zhao, Peng; Zhu, Guijie; Zhang, Weibing; Zhang, Lihua; Liang, Zhen; Zhang, Yukui |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2009 |
| 卷号 | 393期号:1页码:257-261 |
| 关键词 | Binding constant Human serum albumin Dexamethasone Capillary electrophoresis-frontal analysis |
| 产权排序 | 1;1 |
| 通讯作者 | 张丽华 |
| 英文摘要 | studies into the interactions between drugs and human serum albumin (hsa) are extremely important for drug discovery, since hsa behaves as a carrier for external drugs and internal biological molecules. in this paper, to evaluate the pharmacokinetic and pharmacodynamic properties of dexamethasone (dxm), the interaction between dxm and hsa was studied by capillary electrophoresis frontal analysis (ce-fa). according to the klotz equation, four binding sites between dxm and hsa were obtained, and the average binding constant was 1.05 x 10(3) m(-1). furthermore, according to multiple equilibrium theory, based on the assumption that there are two types of binding site, the binding constant at one site was calculated to be 3.539 x 10(3) m(-1), and the average of the other three was 1.234 x 10(3) m(-1). in addition, to obtain the detailed binding information at each binding site, new equations were deduced by multivariate regression. the four binding constants of dxm and hsa were calculated to be 5.558 x 10(1) m(-1), 2.158 x 10(4) m(-1), 7.312 x 10(3) m(-1) and 2.043 x 10(3) m(-1), respectively, which is helpful for detailed studies into the interactions between drugs and proteins with multiple binding sites. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | contactless conductivity detection ; protein interactions ; noncovalent interactions ; drug ; microdialysis ; sites ; uv |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000261831000029 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/101555]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Peng,Zhu, Guijie,Zhang, Weibing,et al. Study of multiple binding constants of dexamethasone with human serum albumin by capillary electrophoresis-frontal analysis and multivariate regression[J]. analytical and bioanalytical chemistry,2009,393(1):257-261. |
| APA | Zhao, Peng,Zhu, Guijie,Zhang, Weibing,Zhang, Lihua,Liang, Zhen,&Zhang, Yukui.(2009).Study of multiple binding constants of dexamethasone with human serum albumin by capillary electrophoresis-frontal analysis and multivariate regression.analytical and bioanalytical chemistry,393(1),257-261. |
| MLA | Zhao, Peng,et al."Study of multiple binding constants of dexamethasone with human serum albumin by capillary electrophoresis-frontal analysis and multivariate regression".analytical and bioanalytical chemistry 393.1(2009):257-261. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。