Developmental neurotoxicity role of cyclophosphamide on post-neural tube closure of rodents in vitro and in vivo
文献类型：期刊论文
| 作者 | Xiao, R.1; Yu, Huan L.1; Zhao, Hai F.2; Liang, J.2; Feng, Jin F.1; Wang, W.1,3 |
| 刊名 | International journal of developmental neuroscience
 |
| 出版日期 | 2007-12-01 |
| 卷号 | 25期号:8页码:531-537 |
| 关键词 | Developmental neurotoxicity Post-neural tube closure Cyclophosphamide Flow cytometry Electron microscope Single cell gel electrophoresis |
| ISSN号 | 0736-5748 |
| DOI | 10.1016/j.ijdevneu.2007.09.012 |
| 通讯作者 | Xiao, r.(xiaor22@ccmu.edu.cn) |
| 英文摘要 | To evaluate the possible developmental role of cyclophosphamide on post-neural tube closure of rodents, the 46 pregnant rats of 2.5-3 months old were randomly divided into five groups. on the 13th day of gestation, the rats in control and experimental groups were treated with physiological saline and cyclophosphamide (7.5, 10.5, 12.5 and 15.0 mg/kg bw), respectively. on the 14th day of gestation; three rats each selected randomly from control and experimental (12.5 mg/kg bw) group, respectively, were executed and the embryo brains were checked using both light microscope and transmission electron microscope. neurocytotoxic effects of cyclophosphamide were determined using tetrazolium dye (mtt) assay, single cell gel electrophoresis, flow cytometry and scanning electron microscope. compared with control group, the malformation incidence of the experimental groups (except the group 7.5 mg/kg bw) was significantly higher, especially in 12.5 mg/kg bw group (malformation incidence 98%, lethality 0). neural tube serial section of fetus showed the apoptotic morphological features after 24 h cyclophosphamide administrated. cyclophosphamide (8 mu g/ml) decreased the growth and viability of neurons, damaged nuclear dna and induced early apoptotic morphological changes. our study shows that the teratogenesis of cyclophosphamide after neural tube closure are involved in cyclophosphamide-induced neuronal apoptosis and dna damage. (c) 2007 published by elsevier ltd on.behalf of isdn. |
| WOS关键词 | LIPOIC ACID ; DNA-DAMAGE ; RAT ; APOPTOSIS ; DEFECTS ; CELLS ; EXPRESSION ; ALPHA ; GENE ; MICE |
| WOS研究方向 | Developmental Biology ; Neurosciences & Neurology |
| WOS类目 | Developmental Biology ; Neurosciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000252164900006 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380304 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Xiao, R. |
| 作者单位 | 1.Capital Med Univ, Sch Publ Hlth & Family Med, Beijing 100069, Peoples R China 2.Shanxi Med Univ, Sch Publ Hlth, Taiyuan 030001, Shanxi, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100054, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiao, R.,Yu, Huan L.,Zhao, Hai F.,et al. Developmental neurotoxicity role of cyclophosphamide on post-neural tube closure of rodents in vitro and in vivo[J]. International journal of developmental neuroscience,2007,25(8):531-537. |
| APA | Xiao, R.,Yu, Huan L.,Zhao, Hai F.,Liang, J.,Feng, Jin F.,&Wang, W..(2007).Developmental neurotoxicity role of cyclophosphamide on post-neural tube closure of rodents in vitro and in vivo.International journal of developmental neuroscience,25(8),531-537. |
| MLA | Xiao, R.,et al."Developmental neurotoxicity role of cyclophosphamide on post-neural tube closure of rodents in vitro and in vivo".International journal of developmental neuroscience 25.8(2007):531-537. |
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来源：中国科学院大学
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