Isolation and functional characterization of pgtip1 a hormone-autotrophic cells-specific tonoplast aquaporin in ginseng
文献类型：期刊论文
| 作者 | Lin, Wuling; Peng, Yanhui; Li, Guowei; Arora, Rajeev; Tang, Zhangcheng; Su, Weiai; Cai, Weiming |
| 刊名 | Journal of experimental botany
 |
| 出版日期 | 2007 |
| 卷号 | 58期号:5页码:947-956 |
| 关键词 | Arabidopsis Ginseng Habituation pgtip1 Tonoplast intrinsic protein |
| ISSN号 | 0022-0957 |
| DOI | 10.1093/jxb/erl255 |
| 通讯作者 | Cai, weiming(wmcai@sippe.ac.cn) |
| 英文摘要 | The suppression subtractive hybridization technique was used to identify differentially expressed genes between hormone-autotrophic and hormone-dependent panax ginseng callus lines. a tonoplast intrinsic protein cdna (pgtip1) was found to be highly and specifically expressed in hormone-autotrophic ginseng cells, which was slightly up-regulated by cytokinin while significantly down-regulated when treated with auxin. pgtip1 encodes a polypeptide of 250 amino acids which shows sequence and structure similarity with tonoplast aquaporins in plants. the water channel activity of pgtip1 was demonstrated by its expression in xenopus laevis oocytes. when over-expressed in arabidopsis thaliana, pgtip1 substantially altered the plant's vegetative and reproductive growth and development. arabidopsis plants over-expressing pgtip1 showed significantly enhanced seed size and seed mass plus greatly increased growth rate compared with those of the wild type. moreover, the seeds from pgtip1 over-expressing arabidopsis had 1.85-fold higher fatty acid content than the wild-type control. these results demonstrate a significant function of pgtip1 in the growth and development of plant cells. |
| WOS关键词 | CYTOKININ SIGNAL-TRANSDUCTION ; MAJOR INTRINSIC PROTEINS ; ROOT WATER-UPTAKE ; PHYSIOLOGICAL FUNCTIONS ; ARABIDOPSIS-THALIANA ; HERITABLE VARIATION ; MEMBRANE AQUAPORIN ; PLASMA-MEMBRANE ; PLANT-CELLS ; EXPRESSION |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000246208900007 |
| 出版者 | OXFORD UNIV PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380305 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Cai, Weiming |
| 作者单位 | 1.Chinese Acad Sci, Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Shanghai 200032, Peoples R China 3.Iowa State Univ, Dept Hort, Ames, IA 50011 USA |
| 推荐引用方式 GB/T 7714 | Lin, Wuling,Peng, Yanhui,Li, Guowei,et al. Isolation and functional characterization of pgtip1 a hormone-autotrophic cells-specific tonoplast aquaporin in ginseng[J]. Journal of experimental botany,2007,58(5):947-956. |
| APA | Lin, Wuling.,Peng, Yanhui.,Li, Guowei.,Arora, Rajeev.,Tang, Zhangcheng.,...&Cai, Weiming.(2007).Isolation and functional characterization of pgtip1 a hormone-autotrophic cells-specific tonoplast aquaporin in ginseng.Journal of experimental botany,58(5),947-956. |
| MLA | Lin, Wuling,et al."Isolation and functional characterization of pgtip1 a hormone-autotrophic cells-specific tonoplast aquaporin in ginseng".Journal of experimental botany 58.5(2007):947-956. |
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来源：中国科学院大学
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