Sample preparation for two-dimensional blue native/sds polyacrylamide gel electrophoresis in the identification of streptomyces coelicolor cytoplasmic protein complexes
文献类型：期刊论文
| 作者 | Wang, Zhi-Jun; Xu, Xin-Ping; Fan, Ke-Qiang; Jia, Cui-Juan; Yang, Ke-Qian |
| 刊名 | Journal of biochemical and biophysical methods
 |
| 出版日期 | 2007-06-10 |
| 卷号 | 70期号:4页码:565-572 |
| ISSN号 | 0165-022X |
| 关键词 | Bn-page Ammonium sulfate precipitation Peptide mass fingerprint Protein identification Streptomyces coelicolor |
| DOI | 10.1016/j.jbbm.2007.01.001 |
| 通讯作者 | Yang, ke-qian(yangkq@im.ac.cn) |
| 英文摘要 | Ammonium sulfate precipitation was tested as a sample preparation step for bn-page analyses of s. coelicolor cytoplasmic protein complexes. a procedure of sample preparation compatible with two-dimensional bn/sds-page was established and used to visualize protein complexes. to validate the sample preparation procedure, representative protein complexes were identified. several previously characterized protein complexes were rediscovered and their reported oligomeric states reconfirmed. in addition, we identified new but plausible interactions that have never been reported before. our work provides useful reference for the wide application of bn-page in protein interaction study. (c) 2007 elsevier b.v. all rights reserved. |
| WOS关键词 | CONTAINING SUPEROXIDE-DISMUTASE ; CRYSTAL-STRUCTURE ; TRANSFER-RNA ; OXIDATIVE-PHOSPHORYLATION ; ANGSTROM RESOLUTION ; MEMBRANE ; DEHYDROGENASE ; A3(2) ; ENZYMES ; NICKEL |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000246933400005 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380309 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yang, Ke-Qian |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Zhi-Jun,Xu, Xin-Ping,Fan, Ke-Qiang,et al. Sample preparation for two-dimensional blue native/sds polyacrylamide gel electrophoresis in the identification of streptomyces coelicolor cytoplasmic protein complexes[J]. Journal of biochemical and biophysical methods,2007,70(4):565-572. |
| APA | Wang, Zhi-Jun,Xu, Xin-Ping,Fan, Ke-Qiang,Jia, Cui-Juan,&Yang, Ke-Qian.(2007).Sample preparation for two-dimensional blue native/sds polyacrylamide gel electrophoresis in the identification of streptomyces coelicolor cytoplasmic protein complexes.Journal of biochemical and biophysical methods,70(4),565-572. |
| MLA | Wang, Zhi-Jun,et al."Sample preparation for two-dimensional blue native/sds polyacrylamide gel electrophoresis in the identification of streptomyces coelicolor cytoplasmic protein complexes".Journal of biochemical and biophysical methods 70.4(2007):565-572. |
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来源：中国科学院大学
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