The bean pvsr2 gene produces two transcripts by alternative promoter usage
文献类型：期刊论文
| 作者 | Qi, Xiao-Ting; Zhang, Yu-Xiu; Chai, Tuan-Yao |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2007-04-27 |
| 卷号 | 356期号:1页码:273-278 |
| 关键词 | Alternative polyadenylation site Heavy metal Intron Intronic promoter |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2007.02.124 |
| 通讯作者 | Chai, tuan-yao(tychai@gucas.ac.cn) |
| 英文摘要 | The bean (phaseolus vulgaris) stress-related gene number 2 (pvsr2) is heavy metal-inducible. here, the intron of pvsr2 (i-pvsr) within the coding sequence was isolated and characterized. i-pvsr exhibited a weak and constitutive promoter activity and enhanced the pvsr2 promoter activity in transiently transformed tobacco protoplasts. the transcription start site of i-pvsr promoter was mapped 72 bp upstream of the 3'-splice site. the shorter pvsr2 transcript (768 nt) in bean is generated from this intronic promoter and lacks the last 56 bases of 3'-end sequence of longer pvsr2 transcript (829 nt) by utilizing an alternative polyadenylation site. quantitative competitive pcr analysis further revealed that two transcripts were differently accumulated in response to hg2+-exposure and the longer transcript was more abundant than the shorter one. these results demonstrate an additional non-metal inducible transcription of pvsr2 via alternative intronic promoter usage and provide new insights into expression mechanism of metal inducible gene. (c) 2007 elsevier inc. all rights reserved. |
| WOS关键词 | HEAVY-METAL ; EXPRESSION ; INTRON ; PLANTS ; CADMIUM ; REGIONS ; TOBACCO |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000245419300046 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380318 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chai, Tuan-Yao |
| 作者单位 | 1.Grad Univ, Chinese Acad Sci, Dept Biol, Beijing 100049, Peoples R China 2.China Univ Mining & Technol, Sch Chem & Environm Engn, Dept Bioengn, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Xiao-Ting,Zhang, Yu-Xiu,Chai, Tuan-Yao. The bean pvsr2 gene produces two transcripts by alternative promoter usage[J]. Biochemical and biophysical research communications,2007,356(1):273-278. |
| APA | Qi, Xiao-Ting,Zhang, Yu-Xiu,&Chai, Tuan-Yao.(2007).The bean pvsr2 gene produces two transcripts by alternative promoter usage.Biochemical and biophysical research communications,356(1),273-278. |
| MLA | Qi, Xiao-Ting,et al."The bean pvsr2 gene produces two transcripts by alternative promoter usage".Biochemical and biophysical research communications 356.1(2007):273-278. |
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来源：中国科学院大学
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