Preparation of monodisperse immobilized Ti4+ affinity chromatography microspheres for specific enrichment of phosphopeptides
文献类型：期刊论文
| 作者 | Yu, Zhiyuan; Han, Guanghui; Sun, Shutao; Jiang, Xinning; Chen, Rui; Wang, Fangjun; Wu, Ren'an; Ye, Mingliang; Zou, Hanfa |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2009-03-16 |
| 卷号 | 636期号:1页码:34-41 |
| 关键词 | Monodisperse Microspheres Phosphopeptides enrichment Phosphoproteome |
| ISSN号 | 0003-2670 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | this study presented an approach to prepare monodisperse immobilized ti4+ affinity chromatography (ti4+-imac) microspheres for specific enrichment of phosphopeptides in phosphoproteome analysis. monodisperse polystyrene seed microspheres with a diameter of ca. 4.8 mu m were first prepared by a dispersion polymerization method. monodisperse microspheres with a diameter of ca. 13 mu m were prepared using the seed microspheres by a single-step swelling and polymerization method. ti4+ ion was immobilized after chemical modification of the microspheres with phosphonate groups. the specificity of the ti4+-imac microspheres to phosphopeptides was demonstrated by selective enrichment of phosphopeptides from mixture of tryptic digests of alpha-casein and bovine serum albumin (bsa) at molar ratio of 1 to 500 by maldi-tof ms analysis. the sensitivity of detection for phosphopeptides determined by maldi-tof ms was as low as 5 fmol for standard tryptic digest of beta-casein. the ti4+-imac microspheres were compared with commercial fe3+-imac adsorbent and homemade zr4+-imac microspheres for enrichment of phosphopeptides. the phosphopeptides and non-phosphopeptides identified by fe3+-imac, zr4+-imac and ti4+-imac methods were 26,114,127 and 181,11,11 respectively for the same tryptic digest samples. the results indicated that the ti4+-imac had the best performance for enrichment of phosphopepticles. (c) 2009 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | tandem mass-spectrometry ; highly selective enrichment ; core-shell microspheres ; linear ion-trap ; tof ms analysis ; phosphoproteome analysis ; phosphorylated peptides ; poly(glycidylmethacrylate-co-ethylenedimethacrylate) beads ; liquid-chromatography ; polymer particles |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000264212100006 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/101567]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Zhiyuan,Han, Guanghui,Sun, Shutao,et al. Preparation of monodisperse immobilized Ti4+ affinity chromatography microspheres for specific enrichment of phosphopeptides[J]. analytica chimica acta,2009,636(1):34-41. |
| APA | Yu, Zhiyuan.,Han, Guanghui.,Sun, Shutao.,Jiang, Xinning.,Chen, Rui.,...&Zou, Hanfa.(2009).Preparation of monodisperse immobilized Ti4+ affinity chromatography microspheres for specific enrichment of phosphopeptides.analytica chimica acta,636(1),34-41. |
| MLA | Yu, Zhiyuan,et al."Preparation of monodisperse immobilized Ti4+ affinity chromatography microspheres for specific enrichment of phosphopeptides".analytica chimica acta 636.1(2009):34-41. |
入库方式： OAI收割
来源：大连化学物理研究所
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