Systematic identification of non-coding rna 2,2,7-trimethylguanosine cap structures in caenorhabditis elegans
文献类型：期刊论文
| 作者 | Jia, Dong1,2; Cai, Lun3,4; He, Housheng1,4; Skogerbo, Geir1; Li, Tiantian1,4; Aftab, Muhammad Nauman1,4; Chen, Runsheng1 |
| 刊名 | Bmc molecular biology
 |
| 出版日期 | 2007-09-29 |
| 卷号 | 8页码:9 |
| ISSN号 | 1471-2199 |
| DOI | 10.1186/1471-2199-8-86 |
| 通讯作者 | Chen, runsheng(crs@sun5.ibp.ac.cn) |
| 英文摘要 | Background: the 2,2,7-trimethylguanosine (tmg) cap structure is an important functional characteristic of ncrnas with critical cellular roles, such as some snrnas. here we used immunoprecipitation with both k121 and r1131 anti-tmg antibodies to systematically identify the tmg cap structures for all presently characterized ncrnas in c. elegans. results: the two anti-tmg antibodies precipitated a similar group of the c. elegans ncrnas. all snrnas known to have a tmg cap structure were found in the precipitate, indicating that our identification system was efficient. other ncrna families related to splicing, such as sl rnas and sm y rnas, were also found in the precipitate, as were 7 c/d box snornas. further analysis showed that the sl rnas and the sm y rnas shared a very similar sm binding site element ( aau(4)-(5)gga), which sequence composition differed somewhat from those of other u snrnas. there were also 16 ncrnas without an sm binding site element in the precipitate, suggesting that for these ncrnas, tmg formation may occur independently of sm proteins. conclusion: our results showed that most ncrnas predicted to be transcribed by rna polymerase ii had a tmg cap, while those predicted to be transcribed by rna plymerase iii or located in introns did not have a tmg cap structure. compared to ncrnas without a tmg cap, tmg-capped ncrnas tended to have higher expression levels. five functionally non-annotated ncrnas also have a tmg cap structure, which might be helpful for identifying the cellular roles of these ncrnas. |
| WOS关键词 | SMALL NUCLEAR RNAS ; RIBONUCLEOPROTEIN-PARTICLES ; SACCHAROMYCES-CEREVISIAE ; POLYMERASE-III ; SPLICED LEADER ; MESSENGER-RNAS ; GUIDE RNAS ; SNRNA ; U1 ; DOMAIN |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| 出版者 | BIOMED CENTRAL LTD |
| WOS记录号 | WOS:000252379900001 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380331 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chen, Runsheng |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Bioinformat Lab, Beijing 100101, Peoples R China 2.Shanxi Agr Univ, Taigu 030801, Peoples R China 3.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc, Beijing 100080, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jia, Dong,Cai, Lun,He, Housheng,et al. Systematic identification of non-coding rna 2,2,7-trimethylguanosine cap structures in caenorhabditis elegans[J]. Bmc molecular biology,2007,8:9. |
| APA | Jia, Dong.,Cai, Lun.,He, Housheng.,Skogerbo, Geir.,Li, Tiantian.,...&Chen, Runsheng.(2007).Systematic identification of non-coding rna 2,2,7-trimethylguanosine cap structures in caenorhabditis elegans.Bmc molecular biology,8,9. |
| MLA | Jia, Dong,et al."Systematic identification of non-coding rna 2,2,7-trimethylguanosine cap structures in caenorhabditis elegans".Bmc molecular biology 8(2007):9. |
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来源：中国科学院大学
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