Nitric oxide controls nuclear export of ape1/ref-1 through s-nitrosation of cysteines 93 and 310
文献类型：期刊论文
| 作者 | Qu, Jing; Liu, Guang-Hui; Huang, Bo; Chen, Chang |
| 刊名 | Nucleic acids research
 |
| 出版日期 | 2007-04-01 |
| 卷号 | 35期号:8页码:2522-2532 |
| ISSN号 | 0305-1048 |
| DOI | 10.1093/nar/gkl1163 |
| 通讯作者 | Chen, chang(changchen@moon.ibp.ac.cn) |
| 英文摘要 | Apurinic/apyrimidinic endonuclease 1/redox effector factor-1 (ape1/ref-1, abbreviated as ape1) is a molecule with dual functions in dna repair and redox regulation of transcription factors. accumulated work has shown that the biological activities of ape1 are sensitive to oxidative stress; however, whether ape1 functions can be regulated by nitrosative stress remains unknown. in this investigation, we found that s-nitrosoglutathion (gsno), a nitric oxide donor and also an s-nitrosating agent, effectively stimulated nuclear export of ape1 in a crm1-independent manner. this nuclear-cytoplasmic translocation was dependent on s-nitrosation modification of ape1, as simultaneous mutation of s-nitrosation target sites cys93 and cys310 completely abrogated the cytoplasmic redistribution. the translocation process was reversal and specific, as it could be reversed by reductive reagents, but could not be mimicked by h2o2. in structure, the region aa. 64-80 and the beta-strand aa.311-316 in proximity to cys93 and cys310 were important for gsno-induced ape1 relocalization. in addition, a defect of importin-mediated nuclear import pathway was found in the no-insulted cells, and p50 and hdac2 were identified as ape1 nuclear export inhibitory proteins. together, this study may provide a novel molecular mechanism, which links nitrosative stress to ape1-associated physiological and pathological processes. |
| WOS关键词 | DNA-REPAIR ENZYME ; HUMAN APURINIC/APYRIMIDINIC ENDONUCLEASE ; TRANSCRIPTIONAL ACTIVITY ; APOPTOTIC FUNCTIONS ; GENE-TRANSCRIPTION ; PROTEIN APE/REF-1 ; OXIDATIVE STRESS ; REDOX REGULATION ; MAMMALIAN-CELLS ; ABASIC SITES |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000247239600007 |
| 出版者 | OXFORD UNIV PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380332 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chen, Chang |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qu, Jing,Liu, Guang-Hui,Huang, Bo,et al. Nitric oxide controls nuclear export of ape1/ref-1 through s-nitrosation of cysteines 93 and 310[J]. Nucleic acids research,2007,35(8):2522-2532. |
| APA | Qu, Jing,Liu, Guang-Hui,Huang, Bo,&Chen, Chang.(2007).Nitric oxide controls nuclear export of ape1/ref-1 through s-nitrosation of cysteines 93 and 310.Nucleic acids research,35(8),2522-2532. |
| MLA | Qu, Jing,et al."Nitric oxide controls nuclear export of ape1/ref-1 through s-nitrosation of cysteines 93 and 310".Nucleic acids research 35.8(2007):2522-2532. |
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来源：中国科学院大学
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