Determination of luteolin and quercetin in the capsule of lamiophlomis rotata (benth.) kudo by hplc coupled with weighted least squares linear regression
文献类型：期刊论文
| 作者 | Wang, Shuai; Di, Duolong; Liu, Xia; Jiang, Shengxiang |
| 刊名 | Journal of liquid chromatography & related technologies
 |
| 出版日期 | 2007 |
| 卷号 | 30期号:13-16页码:1991-1999 |
| 关键词 | Lamiophlomis rotata (benth.) kudo Capsule Luteolin Quercetin Hplc |
| ISSN号 | 1082-6076 |
| DOI | 10.1080/10826070701386637 |
| 通讯作者 | Wang, shuai(s-x-jiang@hotmail.com) |
| 英文摘要 | A simple, rapid and accurate high performance liquid chromatographic (hplc) technique was developed for the simultaneous determination of luteolin and quercetin in the capsule of lamiophlomis rotata (benth.) kudo. separation of luteolin and quercetin was achieved within 20 min using a gradient of water/acetic acid (100/1, v/v) and methanol. by using weighted least squares linear regression (wlslr), we could obtained better linear relationships for luteolin and quercetin in the range of 0.06 similar to 6.00 mu g and 0.064 similar to 6.40 mu g, respectively, compared with the results obtained by using least squares linear regression (lslr). the detection limits for luteolin and quercetin were 7.5 ng and 16.0 ng, at a signal-to-noise ratio of 3, respectively. the mean recovery for luteolin and quercetin was 96.8% and 92.6%, respectively, and rsd was less than 2.0% (n = 3). |
| WOS关键词 | FLAVONOIDS ; IDENTIFICATION ; CHROMATOGRAPHY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000248539300011 |
| 出版者 | TAYLOR & FRANCIS INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380363 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Shuai |
| 作者单位 | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Shuai,Di, Duolong,Liu, Xia,et al. Determination of luteolin and quercetin in the capsule of lamiophlomis rotata (benth.) kudo by hplc coupled with weighted least squares linear regression[J]. Journal of liquid chromatography & related technologies,2007,30(13-16):1991-1999. |
| APA | Wang, Shuai,Di, Duolong,Liu, Xia,&Jiang, Shengxiang.(2007).Determination of luteolin and quercetin in the capsule of lamiophlomis rotata (benth.) kudo by hplc coupled with weighted least squares linear regression.Journal of liquid chromatography & related technologies,30(13-16),1991-1999. |
| MLA | Wang, Shuai,et al."Determination of luteolin and quercetin in the capsule of lamiophlomis rotata (benth.) kudo by hplc coupled with weighted least squares linear regression".Journal of liquid chromatography & related technologies 30.13-16(2007):1991-1999. |
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来源：中国科学院大学
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