Efficient solubilization, purification of recombinant extracellular alpha-amylase from pyrococcus furiosus expressed as inclusion bodies in escherichia coli
文献类型：期刊论文
| 作者 | Wang, Lisa; Zhou, Qinhua; Chen, Huayou; Chu, Zhongmei; Lu, Jianfeng; Zhang, Yi; Yang, Shengli |
| 刊名 | Journal of industrial microbiology & biotechnology
 |
| 出版日期 | 2007-03-01 |
| 卷号 | 34期号:3页码:187-192 |
| 关键词 | Pyrococcus furiosus Escherichia coli Alpha-amylase Inclusion bodies Purification Thermal stability |
| ISSN号 | 1367-5435 |
| DOI | 10.1007/s10295-006-0185-1 |
| 通讯作者 | Zhang, yi(yzhang@sibs.ac.cn) |
| 英文摘要 | The gene encoding the pyrococcus furiosus extracellular alpha-amylase (pfa) was amplified by pcr from p. furiosus genomic dna and was highly expressed in escherichia coli bl21-codon plus (de3)-ril. the recombinant alpha-amylase was mainly expressed in the form of insoluble inclusion bodies. an improved purification method was established in this paper. the solubilization of the inclusion bodies was achieved by 90 degrees c treatment for 3 min in britton-robinson buffer at ph 10.5. the solubilized pfa was then diluted and subsequently purified by phenyl sepharose chromatography. the overall yield of the new purification method was about 58,000 u/g wet cells, which is higher than previously reported. |
| WOS关键词 | BIOCHEMICAL-CHARACTERIZATION ; GROWTH-HORMONE ; CLONING ; ENZYME ; PROTEIN ; GENE ; WOESEI |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000244066500002 |
| 出版者 | SPRINGER HEIDELBERG |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380389 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhang, Yi |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai 200233, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Jiaxing Univ, Coll Med, Jiaxing 314001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Lisa,Zhou, Qinhua,Chen, Huayou,et al. Efficient solubilization, purification of recombinant extracellular alpha-amylase from pyrococcus furiosus expressed as inclusion bodies in escherichia coli[J]. Journal of industrial microbiology & biotechnology,2007,34(3):187-192. |
| APA | Wang, Lisa.,Zhou, Qinhua.,Chen, Huayou.,Chu, Zhongmei.,Lu, Jianfeng.,...&Yang, Shengli.(2007).Efficient solubilization, purification of recombinant extracellular alpha-amylase from pyrococcus furiosus expressed as inclusion bodies in escherichia coli.Journal of industrial microbiology & biotechnology,34(3),187-192. |
| MLA | Wang, Lisa,et al."Efficient solubilization, purification of recombinant extracellular alpha-amylase from pyrococcus furiosus expressed as inclusion bodies in escherichia coli".Journal of industrial microbiology & biotechnology 34.3(2007):187-192. |
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来源：中国科学院大学
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