Insulin rescues es cell-derived neural progenitor cells from apoptosis by differential regulation of akt and erk pathways
文献类型：期刊论文
| 作者 | Zhao, Yanhong1,2; Xiao, Zhifeng1; Gao, Yuan1,2; Chen, Bing1; Zhao, Yannan1,2; Zhang, Jing1,2; Dai, Jianwu1 |
| 刊名 | Neuroscience letters
 |
| 出版日期 | 2007-12-11 |
| 卷号 | 429期号:1页码:49-54 |
| 关键词 | Es-npc Hypoxia Insulin Pi3k Akt Erk |
| ISSN号 | 0304-3940 |
| DOI | 10.1016/j.neulet.2007.09.076 |
| 通讯作者 | Dai, jianwu(jwdai@genetics.ac.cn) |
| 英文摘要 | Transplantation of embryonic stem (es) cell-derived neural progenitor cells (es-npcs) is one promising technology for the treatment of spinal cord injury. promoting es-npc survival at the lesion site is critical for the successful treatment. we tested the role of insulin in promoting mouse es-npc survival. cultured es-npcs survived when maintained in normoxia but underwent apoptosis when exposed to hypoxia. insulin rescued es-npcs from hypoxia-induced cell death. this effect could be blocked by the phosphatidylinositol 3-kinase (pi3k)/akt pathway inhibitor ly294002. in contrast, mitogen-activated protein kinase (map)/extracellular-signal-regulated kinase (erk) pathway inhibitor u0126 potentiated insulin-mediated survival. immunoblots revealed that insulin upregulated activation of akt and inhibited erk activation through the pi3k pathway. in addition, we showed that insulin reduced the activation of caspase-3, the key executor of apoptosis. in summary, our data suggest that insulin prevent apoptosis in es-npcs by activating akt and inhibiting erk through the pi3k pathway. (c) 2007 elsevier ireland ltd. all rights reserved. |
| WOS关键词 | SPINAL-CORD-INJURY ; EMBRYONIC STEM-CELLS ; NEURONAL SURVIVAL ; GROWTH-FACTOR ; IN-VITRO ; KINASE ; BRAIN |
| WOS研究方向 | Neurosciences & Neurology |
| WOS类目 | Neurosciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000251964600010 |
| 出版者 | ELSEVIER IRELAND LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380414 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Dai, Jianwu |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Mol Dev Biol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Yanhong,Xiao, Zhifeng,Gao, Yuan,et al. Insulin rescues es cell-derived neural progenitor cells from apoptosis by differential regulation of akt and erk pathways[J]. Neuroscience letters,2007,429(1):49-54. |
| APA | Zhao, Yanhong.,Xiao, Zhifeng.,Gao, Yuan.,Chen, Bing.,Zhao, Yannan.,...&Dai, Jianwu.(2007).Insulin rescues es cell-derived neural progenitor cells from apoptosis by differential regulation of akt and erk pathways.Neuroscience letters,429(1),49-54. |
| MLA | Zhao, Yanhong,et al."Insulin rescues es cell-derived neural progenitor cells from apoptosis by differential regulation of akt and erk pathways".Neuroscience letters 429.1(2007):49-54. |
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来源：中国科学院大学
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