Trehalose as a good candidate for enriching full-length cdnas in cdna library construction
文献类型：期刊论文
| 作者 | Chen, Lei; Cao, Lixue; Zhou, Longhai; Jing, Yudong; Chen, Zuozhou; Deng, Cheng; Shen, Yu; Chen, Liangbiao |
| 刊名 | Journal of biotechnology
 |
| 出版日期 | 2007-01-10 |
| 卷号 | 127期号:3页码:402-407 |
| 关键词 | Stratascript rt Reverse transcript Full-length cdna library |
| ISSN号 | 0168-1656 |
| DOI | 10.1016/j.jbiotec.2006.07.033 |
| 通讯作者 | Chen, liangbiao(lbchen@genetics.ac.cn) |
| 英文摘要 | It has been reported that the disaccharide trehalose is capable of increasing the thermostability and thermoactivity of reverse transcriptase, and therefore improving the length of cdna synthesis. however, no test has been done on how the disaccharide trehalose performs in the context of the entire cdna synthesis processes, or whether it can seamlessly integrate into the commercially available cdna synthesis kit. in this report, we optimized a protocol to incorporate trehalose in the stratagene's cdna library construction kit in order to demonstrate great improvement in cdna's length (average length of 1.8 kb in the trehalose group versus 1.0 kb in the control). sequence analysis of the cdna clones showed that the addition of trehalose did not increase the error rate of the rt products but greatly increase the quantity of full-length in cdna library. (c) 2006 elsevier b.v. all rights reserved. |
| WOS关键词 | PCR ; STABILIZATION ; AMPLIFICATION ; EFFICIENCY ; ENZYMES ; CLONES ; PROBES ; YEAST ; RNA |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000243697400008 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380417 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chen, Liangbiao |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 3.Chinese Agr Univ, Coll Anim Med, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Lei,Cao, Lixue,Zhou, Longhai,et al. Trehalose as a good candidate for enriching full-length cdnas in cdna library construction[J]. Journal of biotechnology,2007,127(3):402-407. |
| APA | Chen, Lei.,Cao, Lixue.,Zhou, Longhai.,Jing, Yudong.,Chen, Zuozhou.,...&Chen, Liangbiao.(2007).Trehalose as a good candidate for enriching full-length cdnas in cdna library construction.Journal of biotechnology,127(3),402-407. |
| MLA | Chen, Lei,et al."Trehalose as a good candidate for enriching full-length cdnas in cdna library construction".Journal of biotechnology 127.3(2007):402-407. |
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来源：中国科学院大学
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