Molecular analysis of three new receptor-like kinase genes from hexaploid wheat and evidence for their participation in the wheat hypersensitive response to stripe rust fungus infection
文献类型：期刊论文
| 作者 | Zhou, Huanbin; Li, Shaofang; Deng, Zhiyong; Wang, Xianping; Chen, Tao; Zhang, Jinsong; Chen, Shouyi; Ling, Hongqing; Zhang, Aimin; Wang, Daowen |
| 刊名 | Plant journal
 |
| 出版日期 | 2007-11-01 |
| 卷号 | 52期号:3页码:420-434 |
| 关键词 | Receptor-like kinase Stripe rust Virus-induced gene silencing Barley stripe mosaic virus Wheat |
| ISSN号 | 0960-7412 |
| DOI | 10.1111/j.1365-313x.2007.03246.x |
| 通讯作者 | Wang, daowen(dwwang@genetics.ac.cn) |
| 英文摘要 | Considerable progress has been made in understanding the function of receptor-like kinase (rlk) genes in model plants. however, much less is known about these genes in crop species. here we report the characterization of three new wheat rlk genes (tarlk-r1, 2 and 3). the primary structure of the putative proteins tarlk-r1, 2 and 3 contained a signal peptide, a cysteine-rich extracellular domain, a transmembrane domain, and a predicted intracellular kinase domain. the fusions between tarlk-r1, 2 or 3 and the green fluorescence protein (gfp) were targeted to the plasma membrane; such targeting required the signal peptide, extracellular domain and transmembrane domain. transcription of tarlk-r1, 2 and 3 was found mainly in the green organs, and was regulated by light. transcript levels of tarlk-r1, 2 and 3 increased during the hypersensitive reaction (hr) to stripe rust fungus. in addition, the tarlk-r3 transcript level was also upregulated by abiotic stresses. further experiments revealed that the recombinant kinase domain of tarlk-r3 exhibited auto-phosphorylation activity in vitro. knocking down the transcript levels of tarlk-r1, 2 or 3 individually or all together by virus-induced gene silencing compromised the wheat hr to stripe rust fungus. the demonstration of tarlk-r1, 2 and 3 as positive contributors in the wheat hr to stripe rust fungus suggests a new direction for further functional studies of this important family of rlk genes, and may facilitate the breeding of wheat varieties resistant to stripe rust disease. |
| WOS关键词 | TRITICUM-AESTIVUM L. ; PROTEIN-KINASE ; DISEASE RESISTANCE ; ARABIDOPSIS-THALIANA ; ABSCISIC-ACID ; BARLEY ; FAMILY ; PLANT ; RICE ; ERECTA |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000250298600003 |
| 出版者 | WILEY-BLACKWELL PUBLISHING, INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380425 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Daowen |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Huanbin,Li, Shaofang,Deng, Zhiyong,et al. Molecular analysis of three new receptor-like kinase genes from hexaploid wheat and evidence for their participation in the wheat hypersensitive response to stripe rust fungus infection[J]. Plant journal,2007,52(3):420-434. |
| APA | Zhou, Huanbin.,Li, Shaofang.,Deng, Zhiyong.,Wang, Xianping.,Chen, Tao.,...&Zhang, Xiangqi.(2007).Molecular analysis of three new receptor-like kinase genes from hexaploid wheat and evidence for their participation in the wheat hypersensitive response to stripe rust fungus infection.Plant journal,52(3),420-434. |
| MLA | Zhou, Huanbin,et al."Molecular analysis of three new receptor-like kinase genes from hexaploid wheat and evidence for their participation in the wheat hypersensitive response to stripe rust fungus infection".Plant journal 52.3(2007):420-434. |
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来源：中国科学院大学
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