Preparation and biological evaluation of 2-amino-6-[f-18]fluoro-9-(4-hydroxy-3-hydroxy-methylbutyl) purine (6-[f-18]fpcv) as a novel pet probe for imaging hsv1-tk reporter gene expression
文献类型：期刊论文
| 作者 | Cai, Hancheng; Yin, Duanzhi; Zhang, Lan; Yang, Xlaofeng; Xu, Xiaoyan; Liu, Weiguo; Zheng, Xuesheng; Zhang, Hong; Wang, Jing; Xu, Yuhong |
| 刊名 | Nuclear medicine and biology
 |
| 出版日期 | 2007-08-01 |
| 卷号 | 34期号:6页码:717-725 |
| 关键词 | 6-[f-18]fpcv Hsv1-tk Gene expression Pet |
| ISSN号 | 0969-8051 |
| DOI | 10.1016/j.nucmedbio.2007.06.005 |
| 通讯作者 | Yin, duanzhi(chcbati@yahoo.com.cn) |
| 英文摘要 | Introduction: 2-amino-6-[f-18]fluoro-9-(4-hydroxy-3-hydroxy-methylbutyl) purine (6-[f-18]fpcv) was prepared via a one-step nucleophilic substitution and evaluated as a novel probe for imaging the expression of herpes simplex virus type i thymidine kinase (hsvi-tk) reporter gene. methods: log p of 6-[18f]fpcv was calculated in octanol/phosphate-buffered saline (pbs). stability studies were performed in pbs and bovine serum albumin (bsa). cell uptake was performed at various time points in wild-type cells and transduced cells. for in vivo studies, tumors were grown in nude mice by inoculation with c6 cells, wild type and tk positive. the radiotracer was intravenously injected to animals, and micro-pet imaging was performed. biodistribution of 6-[f-18]fpcv was performed on another group of animals at different time points. results: log p of 6-[f-18]fpcv was -0.517. 6-[f-18]fpcv was fairly stable in pbs and bsa at 6 h. the tracer uptake in c6-tk cells was 5.5-18.8 times higher than that in wild-type cells, the plasma half-life of 6-[f-18]fpcv was as follows: u t(1/2)=1.2 min and 1 t(1/2) = 73.7 min. the average ratio of tumor uptake between the transduced tumor and the wild-type tumor was 1.69 at 15 min. conclusion: biological evaluation showed that 6-[18f]fpcv is a potential probe for imaging hsv1 -tk gene expression. however, its in vivo defluorination may limit its application in pet imaging of gene expression. (c) 2007 elsevier inc. all rights reserved. |
| WOS关键词 | POSITRON-EMISSION-TOMOGRAPHY ; IN-VIVO ; THYMIDINE KINASE ; PYRIMIDINE NUCLEOSIDES ; RADIOTRACER ; THERAPY ; ANALOGS ; CANCER ; VITRO |
| WOS研究方向 | Radiology, Nuclear Medicine & Medical Imaging |
| WOS类目 | Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000249157300016 |
| 出版者 | ELSEVIER SCIENCE INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380472 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yin, Duanzhi |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Appl Phys, Res Ctr Radiopharmaceut, Shanghai 201800, Peoples R China 2.Zhejiang Univ, Affiliated Hosp 2, Sch Med, Inst Brain Med Sci, Hangzhou 310009, Peoples R China 3.Zhejiang Univ, Med Sch, Hangzhou 310009, Peoples R China 4.Zhejiang Calif Int NanoSyst Inst, Hangzhou 310029, Peoples R China 5.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cai, Hancheng,Yin, Duanzhi,Zhang, Lan,et al. Preparation and biological evaluation of 2-amino-6-[f-18]fluoro-9-(4-hydroxy-3-hydroxy-methylbutyl) purine (6-[f-18]fpcv) as a novel pet probe for imaging hsv1-tk reporter gene expression[J]. Nuclear medicine and biology,2007,34(6):717-725. |
| APA | Cai, Hancheng.,Yin, Duanzhi.,Zhang, Lan.,Yang, Xlaofeng.,Xu, Xiaoyan.,...&Wang, Yongxian.(2007).Preparation and biological evaluation of 2-amino-6-[f-18]fluoro-9-(4-hydroxy-3-hydroxy-methylbutyl) purine (6-[f-18]fpcv) as a novel pet probe for imaging hsv1-tk reporter gene expression.Nuclear medicine and biology,34(6),717-725. |
| MLA | Cai, Hancheng,et al."Preparation and biological evaluation of 2-amino-6-[f-18]fluoro-9-(4-hydroxy-3-hydroxy-methylbutyl) purine (6-[f-18]fpcv) as a novel pet probe for imaging hsv1-tk reporter gene expression".Nuclear medicine and biology 34.6(2007):717-725. |
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来源：中国科学院大学
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