Identification and characterization of sanh and sani involved in the hydroxylation of pyridyl residue during nikkomycin biosynthesis in streptomyces ansochromogenes
文献类型：期刊论文
| 作者 | Xie, Zhoujie; Niu, Guoqing; Li, Rui; Liu, Gang; Tan, Huarong |
| 刊名 | Current microbiology
 |
| 出版日期 | 2007-12-01 |
| 卷号 | 55期号:6页码:537-542 |
| ISSN号 | 0343-8651 |
| DOI | 10.1007/s00284-007-9028-1 |
| 通讯作者 | Tan, huarong(tanhr@sun.im.ac.cn) |
| 英文摘要 | Nikkomycins are highly potent inhibitors of chitin synthase. the nikkomycin biosynthetic gene cluster has been cloned from streptomyces asochromogenes. two cytochrome p450 monooxygenase genes (sanq, sanh) and one ferredoxin gene (sani) were found in the cluster. it was reported that sanq is involved in the hydroxylation of l-his, a key step in 4-formyl-4-imidazolin-2-one base biosynthesis. here, we have studied the function of sanh and sani. disruption of sanh abolished the production of nikkomycin x and z, but it accumulated one dominant component nikkomycin lx, which is the nikkomycin x analog lacking the hydroxy group at the pyridyl residue. the sani disruption mutant accumulated predominantly nikkomycin lx in addition to nikkomycin x and z. the nikkomycin production profile of the sanh and sani double disruption mutant was the same as that of the sanh disruption mutant. these results confirmed that sanh is essential for the hydroxylation of pyridyl residue in nikkomycin biosynthesis of s. ansochromogenes and first demonstrated that sani is an effective electron donor for sanh, but not for sanq in vivo. |
| WOS关键词 | NUCLEOSIDE ANTIBIOTIC NIKKOMYCIN ; TENDAE TU901 ; MOLECULAR CHARACTERIZATION ; CYTOCHROME-P450 ; GENE ; CLONING ; MOIETY |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000250407000014 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380525 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Tan, Huarong |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Univ Oklahoma, Hlth Sci Ctr, Coll Dent, Oklahoma City, OK 73104 USA |
| 推荐引用方式 GB/T 7714 | Xie, Zhoujie,Niu, Guoqing,Li, Rui,et al. Identification and characterization of sanh and sani involved in the hydroxylation of pyridyl residue during nikkomycin biosynthesis in streptomyces ansochromogenes[J]. Current microbiology,2007,55(6):537-542. |
| APA | Xie, Zhoujie,Niu, Guoqing,Li, Rui,Liu, Gang,&Tan, Huarong.(2007).Identification and characterization of sanh and sani involved in the hydroxylation of pyridyl residue during nikkomycin biosynthesis in streptomyces ansochromogenes.Current microbiology,55(6),537-542. |
| MLA | Xie, Zhoujie,et al."Identification and characterization of sanh and sani involved in the hydroxylation of pyridyl residue during nikkomycin biosynthesis in streptomyces ansochromogenes".Current microbiology 55.6(2007):537-542. |
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来源：中国科学院大学
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