Serially coupled microcolumn reversed phase liquid chromatography for shotgun proteomic analysis
文献类型：期刊论文
| 作者 | Tao, Dingyin1,2; Zhu, Guajie1,2; Sun, Liangliang1,2; Ma, Junfeng1,2; Liang, Zhen1; Zhang, Weibing1; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | proteomics
 |
| 出版日期 | 2009-04-01 |
| 卷号 | 9期号:7页码:2029-2036 |
| 关键词 | mu RPLC MS/MS Serially coupled microcolumn Shotgun proteome analysis |
| 产权排序 | 1;1 |
| 通讯作者 | 张丽华 |
| 英文摘要 | microcolumn rplc (mu rplc) is one of the optimum separation modes for shotgun proteomic analysis. to identify as many proteins as possible by ms/ms, the improvement on separation efficiency and peak capacity of mu rplc is indispensable. although the increase in column length is one of the effective solutions, the preparation of along microcolumn is rather difficult due to the high backpressure generated during the packing procedure. in our recent work, through connecting microcolumns of 5, 10, and 15 cm length via unions with minimal dead volume, long microcolumns with length up to 30 cm were obtained, with which 318 proteins were identified from proteins extracted from escherichia coli by mu rplc-esi ms/ms, and similar distributions of m(w) and pi were found with single and various coupled microcolumns. furthermore, by using ms/ms with improved sensitivity, with such a serially coupled 30 cm long microcolumn, 1692 proteins were identified within 7 h from rat brain tissue, with false positive rate (fpr) < 1%. all these results demonstrated that serially couple microcolumns might be of great promising to improve the separation capacity of mu rplc in shotgun proteomic analysis. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods ; biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | 2-dimensional gel-electrophoresis ; monolithic silica columns ; tandem mass-spectrometry ; low-abundance proteins ; escherichia-coli ; peak-capacity ; performance ; temperature ; technology ; automation |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000265339000025 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/101655]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tao, Dingyin,Zhu, Guajie,Sun, Liangliang,et al. Serially coupled microcolumn reversed phase liquid chromatography for shotgun proteomic analysis[J]. proteomics,2009,9(7):2029-2036. |
| APA | Tao, Dingyin.,Zhu, Guajie.,Sun, Liangliang.,Ma, Junfeng.,Liang, Zhen.,...&Zhang, Yukui.(2009).Serially coupled microcolumn reversed phase liquid chromatography for shotgun proteomic analysis.proteomics,9(7),2029-2036. |
| MLA | Tao, Dingyin,et al."Serially coupled microcolumn reversed phase liquid chromatography for shotgun proteomic analysis".proteomics 9.7(2009):2029-2036. |
入库方式： OAI收割
来源：大连化学物理研究所
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