Mechanism for the ttdnaa-tt-oric cooperative interaction at high temperature and duplex opening at an unusual at-rich region in thermoanaerobacter tengcongensis
文献类型：期刊论文
| 作者 | Pei, Huadong; Liu, Jingfang; Li, Jie; Guo, Aobo; Zhou, Jian; Xiang, Hua |
| 刊名 | Nucleic acids research
 |
| 出版日期 | 2007-05-01 |
| 卷号 | 35期号:9页码:3087-3099 |
| ISSN号 | 0305-1048 |
| DOI | 10.1093/nar/gkm137 |
| 通讯作者 | Xiang, hua(xiangh@sun.im.ac.cn) |
| 英文摘要 | Thermoanaerobacter tengcongensis is an anaerobic low- gc thermophilic bacterium. to further elucidate the replication initiation of chromosomal dna at high temperature, the interaction between the replication initiator ( ttdnaa) and the putative origin ( tt-oric) in this thermophile was investigated. we found that efficient binding of ttdnaa to tt-oric at high temperature requires ( i) at least two neighboring dnaa boxes, ( ii) the specific feature of the ttdnaa domain iv and ( iii) the self-oligomerization of ttdnaa. replacement of the ttdnaa domain iv by the counterpart of escherichia coli dnaa or disruption of its oligomerization by amino acid mutations ( w9a/l20s) abolished the oric- binding activity of ttdnaa at 60 degrees c, but not at 37 degrees c. moreover, atp- ttdnaa, but not adp- ttdnaa or the oligomerization- deficient mutants was able to unwind the tt- oric duplex. the minimal oric required for this duplex opening in vitro was demonstrated to consist of dnaa boxes 1 - 8 and an unusual at- rich region. interestingly, although no typical atp- dnaa box was found in this at- rich region, it was exclusively bound by atp- ttdnaa and acted as the duplex-opening and replication- initiation site. taken together, we propose that oligomerization of atp- dnaa and simultaneously binding of several dnaa boxes and/ or at- rich region may be generally required in replication initiation at high temperature. |
| WOS关键词 | REPLICATION INITIATOR DNAA ; ESCHERICHIA-COLI ; CHROMOSOMAL ORIGIN ; SEQUENTIAL BINDING ; PROTEIN DNAA ; COMPLEX ; IDENTIFICATION ; HDA ; PURIFICATION ; ARCHITECTURE |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000247350800026 |
| 出版者 | OXFORD UNIV PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380816 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Xiang, Hua |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100080, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pei, Huadong,Liu, Jingfang,Li, Jie,et al. Mechanism for the ttdnaa-tt-oric cooperative interaction at high temperature and duplex opening at an unusual at-rich region in thermoanaerobacter tengcongensis[J]. Nucleic acids research,2007,35(9):3087-3099. |
| APA | Pei, Huadong,Liu, Jingfang,Li, Jie,Guo, Aobo,Zhou, Jian,&Xiang, Hua.(2007).Mechanism for the ttdnaa-tt-oric cooperative interaction at high temperature and duplex opening at an unusual at-rich region in thermoanaerobacter tengcongensis.Nucleic acids research,35(9),3087-3099. |
| MLA | Pei, Huadong,et al."Mechanism for the ttdnaa-tt-oric cooperative interaction at high temperature and duplex opening at an unusual at-rich region in thermoanaerobacter tengcongensis".Nucleic acids research 35.9(2007):3087-3099. |
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来源：中国科学院大学
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