Surface-modified polystyrene beads as photografting imprinted polymer matrix for chromatographic separation of proteins
文献类型：期刊论文
| 作者 | Qin, Lei1; He, Xi-Wen1; Zhang, Wei1; Li, Wen-You1; Zhang, Yu-Kui1,2 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2009-01-30 |
| 卷号 | 1216期号:5页码:807-814 |
| 关键词 | Molecular imprinting Protein separation Polystyrene beads Iniferter Chromatography |
| 通讯作者 | 张玉奎 |
| 产权排序 | 2;5 |
| 英文摘要 | a new and facile fabricating method for lysozyme molecularly imprinted polymer beads (lysozyme-mip beads) in aqueous media was presented, mesoporous chloromethylated polystyrene beads (mcp beads) containing dithiocarbamate iniferter (initiator transfer agent terminator) were used as supports for the grafting of lysozyme imprinted copolymers with acrylamide and n,n'-methylenebisacrylamide through surface initiated living-radical polymerization (sip). after the polymerization, a layer of lysozyme-mip was formed on the mcp beads. the sip allowed an efficient control of the grafting process and suppressed solution propagation. therefore, the obtained lysozyme-mip beads had a large quantity of well-distributed pores on the surface without any visible gel formation in solution and were more advantageous comparing with traditional mips which were prepared by traditionally initiated radical polymerization. the obtained composites were characterized by fourier transform infrared spectroscopy, elemental analysis, nitrogen sorption analysis and scanning electron microscopy. chromatographic behaviors of the column packed with lysozyme-mip beads exhibited ability in separating lysozyme from competitive protein (bovine hemoglobin, bovine serum albumin, ovalbumin or cytochrome c) in aqueous mobile phase. (c) 2008 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | electrostatic functional-groups ; recognition ; copolymerization ; antibodies ; films ; gels |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000262797100008 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/101675]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin, Lei,He, Xi-Wen,Zhang, Wei,et al. Surface-modified polystyrene beads as photografting imprinted polymer matrix for chromatographic separation of proteins[J]. journal of chromatography a,2009,1216(5):807-814. |
| APA | Qin, Lei,He, Xi-Wen,Zhang, Wei,Li, Wen-You,&Zhang, Yu-Kui.(2009).Surface-modified polystyrene beads as photografting imprinted polymer matrix for chromatographic separation of proteins.journal of chromatography a,1216(5),807-814. |
| MLA | Qin, Lei,et al."Surface-modified polystyrene beads as photografting imprinted polymer matrix for chromatographic separation of proteins".journal of chromatography a 1216.5(2009):807-814. |
入库方式： OAI收割
来源：大连化学物理研究所
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