Partial strands synthesizing leads to inevitable aborting and complicated products in consecutive polymerase chain reactions (pcrs)
文献类型：期刊论文
| 作者 | Luo Rui; Zhang DaMing |
| 刊名 | Science in china series c-life sciences
 |
| 出版日期 | 2007-08-01 |
| 卷号 | 50期号:4页码:548-556 |
| 关键词 | Consecutive pcr Abnormal complicated product Partial strand synthesis Disturbing effect Pcr-mediated recombination Long distance pcr |
| ISSN号 | 1006-9305 |
| DOI | 10.1007/s11427-007-0043-z |
| 通讯作者 | Zhang daming(zhangdm@ibcas.ac.cn) |
| 英文摘要 | Various abnormal phenomena have been observed during pcr so far. the present study performed a series of consecutive pcrs (including many rounds of re-amplification continuously) and found that the abortion of re-amplification was inevitable as long as a variety of complicated product appeared. the aborting stages varied, according to the lengths of targets. longer targets reached the abortion earlier than the shorter ones, marked by appearance of the complex that was immobile in electrophoresis. denatured gel-electrophoresis revealed that the complex was mainly made up of shorter or partially synthesized strands, together with small amounts of full-length ones. able to be digested by s1 nuclease but unable by restriction endonucleases (res), the complex was proved to consist of both single regions and double-helix regions that kept the complex stable thermodynamically. simulations gave evidence that partial strands, even at lower concentration, could disturb re-amplification effectively and lead to the abortion of re-amplifications finally. it was pointed out that the partial strands formed chiefly via polymerase's infidelity, and hence the solution to lighten the abnormality was also proposed. |
| WOS关键词 | TAQ DNA-POLYMERASE ; IN-VITRO ; EFFECTIVE AMPLIFICATION ; MEDIATED RECOMBINATION ; DIFFERENTIAL DISPLAY ; PROCESSIVITY ; FRAGMENTS ; REAMPLIFICATION ; EXTENSION ; SEQUENCES |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
| WOS类目 | Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000249267000017 |
| 出版者 | SCIENCE CHINA PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380887 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhang DaMing |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolut Bot, Beijing 100093, Peoples R China 2.Grad Univ, Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo Rui,Zhang DaMing. Partial strands synthesizing leads to inevitable aborting and complicated products in consecutive polymerase chain reactions (pcrs)[J]. Science in china series c-life sciences,2007,50(4):548-556. |
| APA | Luo Rui,&Zhang DaMing.(2007).Partial strands synthesizing leads to inevitable aborting and complicated products in consecutive polymerase chain reactions (pcrs).Science in china series c-life sciences,50(4),548-556. |
| MLA | Luo Rui,et al."Partial strands synthesizing leads to inevitable aborting and complicated products in consecutive polymerase chain reactions (pcrs)".Science in china series c-life sciences 50.4(2007):548-556. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。