Improved protein separation by microchip isoelectric focusing with stepwise gradient of electric field strength
文献类型：期刊论文
| 作者 | Cong, Yongzheng1,2; Liang, Yu1; Zhang, Lihua1; Zhang, Weibing1; Zhang, Yukui1 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2009-02-01 |
| 卷号 | 32期号:3页码:462-465 |
| 关键词 | IEF Microchip Protein separation Stepwise gradient of electric field strength |
| 产权排序 | 1;1 |
| 通讯作者 | 张丽华 |
| 英文摘要 | a stepwise gradient of electric field strength was proposed for microchip ief-based protein separation, by which after focusing at low voltages, ief was performed by applying higher separation voltages step-by-step. a linear relationship between the focusing time and the inverse of the electric field strength was found. in addition, the conductivity of an established ph gradient showed a negative but nonlinear correlation with the applied voltage. based on the above-mentioned results, a stepwise gradient of electric field strength, ranging from 160 to 1500 v/cm was applied in the separation of proteins extracted from escherichia coli in a straight glass microchip channel permanently coated by polyacrylamide. compared to the conventional separation performed under a constant field strength of 750 v/cm, the increased stepwise gradient of electric field strength resulted in improved resolution and decreased focusing time, while without the negative effects of joule heat for protein separation. all these results demonstrated that such a method might be of great significance to achieve high resolution and high-throughput analysis of complex protein samples for microchip ief. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | plastic microfluidic devices ; electrophoresis ; chip ; microchannels ; adaptation |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000263385600018 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/101679]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cong, Yongzheng,Liang, Yu,Zhang, Lihua,et al. Improved protein separation by microchip isoelectric focusing with stepwise gradient of electric field strength[J]. journal of separation science,2009,32(3):462-465. |
| APA | Cong, Yongzheng,Liang, Yu,Zhang, Lihua,Zhang, Weibing,&Zhang, Yukui.(2009).Improved protein separation by microchip isoelectric focusing with stepwise gradient of electric field strength.journal of separation science,32(3),462-465. |
| MLA | Cong, Yongzheng,et al."Improved protein separation by microchip isoelectric focusing with stepwise gradient of electric field strength".journal of separation science 32.3(2009):462-465. |
入库方式： OAI收割
来源：大连化学物理研究所
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