Differential gene expression in an elite hybrid rice cultivar (oryza sativa, l) and its parental lines based on sage data
文献类型：期刊论文
| 作者 | Song, Shuhui; Qu, Hongzhu; Chen, Chen; Hu, Songnian; Yu, Jun |
| 刊名 | Bmc plant biology
 |
| 出版日期 | 2007-09-19 |
| 卷号 | 7页码:15 |
| ISSN号 | 1471-2229 |
| DOI | 10.1186/1471-2229-7-49 |
| 通讯作者 | Song, shuhui(songsh@genomics.org.cn) |
| 英文摘要 | Background: it was proposed that differentially-expressed genes, aside from genetic variations affecting protein processing and functioning, between hybrid and its parents provide essential candidates for studying heterosis or hybrid vigor. based our serial analysis of gene expression (sage) data from an elite chinese super-hybrid rice (lyp9) and its parental cultivars (93-11 and pa64s) in three major tissue types (leaves, roots and panicles) at different developmental stages, we analyzed the transcriptome and looked for candidate genes related to rice heterosis. results: by using an improved strategy of tag-to-gene mapping and two recently annotated genome assemblies (93-11 and pa64s), we identified 10,268 additional high-quality tags, reaching a grand total of 20,595 together with our previous result. we further detected 8.5% and 5.9% physically-mapped genes that are differentially-expressed among the triad (in at least one of the three stages) with p-values less than 0.05 and 0.01, respectively. these genes distributed in 12 major gene expression patterns; among them, 406 up-regulated and 469 down-regulated genes (p < 0.05) were observed. functional annotations on the identified genes highlighted the conclusion that upregulated genes (some of them are known enzymes) in hybrid are mostly related to enhancing carbon assimilation in leaves and roots. in addition, we detected a group of up-regulated genes related to male sterility and 442 down-regulated genes related to signal transduction and protein processing, which may be responsible for rice heterosis. conclusion: we improved tag-to-gene mapping strategy by combining information from transcript sequences and rice genome annotation, and obtained a more comprehensive view on genes that related to rice heterosis. the candidates for heterosis-related genes among different genotypes provided new avenue for exploring the molecular mechanism underlying heterosis. |
| WOS关键词 | FLORAL ORGAN IDENTITY ; TRITICUM-AESTIVUM L. ; SERIAL ANALYSIS ; MESSENGER-RNA ; F-1 HYBRID ; IDENTIFICATION ; HETEROSIS ; PATTERNS ; MAIZE ; LEAF |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000250876700001 |
| 出版者 | BIOMED CENTRAL LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2380999 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Song, Shuhui |
| 作者单位 | 1.Chinese Acad Sci, Beijing Inst Genom, Key Lab Genome Sci & Informat, Beijing 101300, Peoples R China 2.Grad Univ Chinese Acad Sci, Dept Biol, Beijing 100094, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Shuhui,Qu, Hongzhu,Chen, Chen,et al. Differential gene expression in an elite hybrid rice cultivar (oryza sativa, l) and its parental lines based on sage data[J]. Bmc plant biology,2007,7:15. |
| APA | Song, Shuhui,Qu, Hongzhu,Chen, Chen,Hu, Songnian,&Yu, Jun.(2007).Differential gene expression in an elite hybrid rice cultivar (oryza sativa, l) and its parental lines based on sage data.Bmc plant biology,7,15. |
| MLA | Song, Shuhui,et al."Differential gene expression in an elite hybrid rice cultivar (oryza sativa, l) and its parental lines based on sage data".Bmc plant biology 7(2007):15. |
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来源：中国科学院大学
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