Activation of paternally expressed imprinted genes in newly derived germline-competent mouse parthenogenetic embryonic stem cell lines
文献类型：期刊论文
| 作者 | Jiang, Hua; Sun, Bowen; Wang, Weichung; Zhang, Zhihong; Gao, Furong; Shi, Guilai; Cui, Bing; Kong, Xiangyin; He, Zhao; Ding, Xiaoyan |
| 刊名 | Cell research
 |
| 出版日期 | 2007-09-01 |
| 卷号 | 17期号:9页码:792-803 |
| 关键词 | Parthenogenesis Embryonic stem cell Pluripotency Imprinted gene Methylation |
| ISSN号 | 1001-0602 |
| DOI | 10.1038/cr.2007.70 |
| 通讯作者 | Feng, yun(artruijin@yahoo.com.cn) |
| 英文摘要 | Parthenogenetic embryonic stem (pes) cells provide a valuable in vitro model system for studying the molecular mechanisms that underlie genomic imprinting. however, the pluripotency of pes cells and the expression profiles of paternally expressed imprinted genes have not been fully explored. in this study, three mouse pes cell lines were established and the differentiation potential of these cells in extended culture was evaluated. the undifferentiated cells had a normal karyotype and homozygous genome, and expressed es-cell-specific molecular markers. the cells remained undifferentiated after more than 50 passages and exhibited pluripotent differentiation capacity. all three lines of the established es cells produced teratomas; two lines of es cells produced chimeras and germline transmission. furthermore, activation of the paternally expressed imprinted genes snrpn, u2afl-rs1, peg3, impact, zfp127, dlki and mest in these cells was detected. some paternally expressed imprinted genes were found to be expressed in the blastocyst stage of parthenogenetically activated embryos in vitro and their expression level increased with extended pes cell culture. furthennore, our data show that the activation of these paternally expressed imprinted genes in pes cells was associated with a change in the methylation of the related differentially methylated regions. these findings provide direct evidence for the pluripotency of pes cells and demonstrate the association between the dna methylation pattern and the activation of paternally expressed imprinted genes in pes cells. thus, the established es cell lines provide a valuable model for studying epigenetic regulation in mammalian development. |
| WOS关键词 | IN-VITRO DIFFERENTIATION ; METHYLATION ; OOCYTES ; FETUSES ; ESTABLISHMENT ; PATTERNS ; GENOMES ; MICE |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000250454800007 |
| 出版者 | INST BIOCHEMISTRY & CELL BIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2381027 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Feng, Yun |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Sch Med, Chinese Acad Sci, Shanghai Inst Biol Sci,Inst Hlth Sci,Key Lab Stem, Shanghai 200025, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Med, Shanghai Inst Stem Cell Res, Shanghai 200025, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cellular Biol, Shanghai 200031, Peoples R China 5.Shanghai Ctr Model Organisms, Shanghai 201203, Peoples R China 6.Ruijin Hosp, Reprod Med Ctr, Shanghai 20135, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Hua,Sun, Bowen,Wang, Weichung,et al. Activation of paternally expressed imprinted genes in newly derived germline-competent mouse parthenogenetic embryonic stem cell lines[J]. Cell research,2007,17(9):792-803. |
| APA | Jiang, Hua.,Sun, Bowen.,Wang, Weichung.,Zhang, Zhihong.,Gao, Furong.,...&Jin, Ying.(2007).Activation of paternally expressed imprinted genes in newly derived germline-competent mouse parthenogenetic embryonic stem cell lines.Cell research,17(9),792-803. |
| MLA | Jiang, Hua,et al."Activation of paternally expressed imprinted genes in newly derived germline-competent mouse parthenogenetic embryonic stem cell lines".Cell research 17.9(2007):792-803. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。