Genome-wide gene expression profiling reveals conserved and novel molecular functions of the stigma in rice(1[w])
文献类型：期刊论文
| 作者 | Li, Meina; Xu, Wenying; Yang, Wenqiang; Kong, Zhaosheng; Xue, Yongbiao |
| 刊名 | Plant physiology
 |
| 出版日期 | 2007-08-01 |
| 卷号 | 144期号:4页码:1797-1812 |
| ISSN号 | 0032-0889 |
| 通讯作者 | Xue, yongbiao(ybxue@genetics.ac.cn) |
| 英文摘要 | In angiosperms, the stigma provides initial nutrients and guidance cues for pollen grain germination and tube growth. however, little is known about the genes that regulate these processes in rice (oryza sativa). here, we generate rice stigma-specific or -preferential gene expression profiles through comparing genome-wide expression patterns of hand-dissected, unpollinated stigma at anthesis with seven tissues, including seedling shoot, seedling root, mature anther, ovary at anthesis, seeds 5 d after pollination, 10-d-old embryo, 10-d-old endosperm, and suspension-cultured cells by using both 57 k affymetrix rice whole-genome array and 10 k rice cdna microarray. a high reproducibility of the microarray results was detected between the two different technology platforms. in total, we identified 548 genes to be expressed specifically or predominantly in the stigma papillar cells of rice. real-time quantitative reverse transcription-polymerase chain reaction analysis of 34 selected genes all confirmed their stigma-specific expression. the expression of five selected genes was further validated by rna in situ hybridization. gene ontology analysis shows that several auxin-signaling components, transcription, and stress-related genes are significantly overrepresented in the rice stigma gene set. interestingly, most of them also share several cisregulatory elements with known stress-responsive genes, supporting the notion of an overlap of genetic programs regulating pollination and stress/defense responses. we also found that genes involved in cell wall metabolism and cellular communication appear to be conserved in the stigma between rice and arabidopsis (arabidopsis thaliana). our results indicate that the stigmas appear to have conserved and novel molecular functions between rice and |
| WOS关键词 | RICE ORYZA-SATIVA ; POLLEN-TUBE GROWTH ; SELF-INCOMPATIBILITY ; CELL-WALL ; S-LOCUS ; SEXUAL REPRODUCTION ; MICROARRAY ANALYSIS ; PISTIL INTERACTIONS ; FLOWER DEVELOPMENT ; STRESS RESPONSES |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000248704800010 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2381069 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Xue, Yongbiao |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Mol & Dev Biol Lab, Beijing 100080, Peoples R China 2.Natl Ctr Plant Gene Res, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Meina,Xu, Wenying,Yang, Wenqiang,et al. Genome-wide gene expression profiling reveals conserved and novel molecular functions of the stigma in rice(1[w])[J]. Plant physiology,2007,144(4):1797-1812. |
| APA | Li, Meina,Xu, Wenying,Yang, Wenqiang,Kong, Zhaosheng,&Xue, Yongbiao.(2007).Genome-wide gene expression profiling reveals conserved and novel molecular functions of the stigma in rice(1[w]).Plant physiology,144(4),1797-1812. |
| MLA | Li, Meina,et al."Genome-wide gene expression profiling reveals conserved and novel molecular functions of the stigma in rice(1[w])".Plant physiology 144.4(2007):1797-1812. |
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来源：中国科学院大学
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