Stability engineering, biophysical, and biological characterization of the myeloid activating receptor immunoglobulin-like transcript 1 (ilt1/lir-7/lilra2)
文献类型：期刊论文
| 作者 | Chen, Yong; Chu, Fuliang; Gao, Feng; Zhou, Bin; Gao, George F. |
| 刊名 | Protein expression and purification
 |
| 出版日期 | 2007-12-01 |
| 卷号 | 56期号:2页码:253-260 |
| 关键词 | Ilt1 Lir-7 Lilra2 Activating receptor Protein engineering Dimer Monomer Crystallization |
| ISSN号 | 1046-5928 |
| DOI | 10.1016/j.pep.2007.08.010 |
| 通讯作者 | Gao, george f.(gaof@im.ac.cn) |
| 英文摘要 | Immunoglobulin-like transcript 1 (ilt1/lir-7/lilra2/cd85h) is one of the activating receptors in the ilt family whose members have been reported to regulate a broad range of cells involved in the immune response. although inhibitory ilt receptors have been extensively studied, however, functions and structures of ilt activating receptors have yet to be elucidated. obtaining of sufficient amount of recombinant proteins is a requisite for the functional and structural studies of a given protein. as a technical bottleneck of the study, extracellular domains of the ilt1 form aggregation during recombinant production in the past efforts. here, we report the large-scale stable production of ilt1 did2 domains through engineering of site-directed mutagenesis (r142c) that introduces a cysteine at amino acid position 142 to form a disulfide bond with the spare cys132 without topological influences of the native protein based on the known structures of the homologous ilt 2/4/11. the recombinant ilt1 d1d2 domains behave as an equilibrium of both stable dimer and monomer in solution and yield ideal crystals for structural determination. the availability of quantities of soluble ilt1 did2 domains provides useful reagent for further studies of its detailed structure and functions. (c) 2007 elsevier inc. all rights reserved. |
| WOS关键词 | CLASS-I MOLECULES ; CD8 ALPHA-ALPHA ; CRYSTAL-STRUCTURE ; INHIBITORY RECEPTOR ; MODULATE FUNCTION ; HLA-G ; CELLS ; LIGAND ; COMPLEX ; FAMILY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000251312300014 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2381074 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Gao, George F. |
| 作者单位 | 1.Chinese Acad Sci, Ctr Mol Immunol, Inst Microbiol, China Japan Joint Lab Mol Immunol & Mol Microbiol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.China Agr Univ, Coll Life Sci, Beijing 100094, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Yong,Chu, Fuliang,Gao, Feng,et al. Stability engineering, biophysical, and biological characterization of the myeloid activating receptor immunoglobulin-like transcript 1 (ilt1/lir-7/lilra2)[J]. Protein expression and purification,2007,56(2):253-260. |
| APA | Chen, Yong,Chu, Fuliang,Gao, Feng,Zhou, Bin,&Gao, George F..(2007).Stability engineering, biophysical, and biological characterization of the myeloid activating receptor immunoglobulin-like transcript 1 (ilt1/lir-7/lilra2).Protein expression and purification,56(2),253-260. |
| MLA | Chen, Yong,et al."Stability engineering, biophysical, and biological characterization of the myeloid activating receptor immunoglobulin-like transcript 1 (ilt1/lir-7/lilra2)".Protein expression and purification 56.2(2007):253-260. |
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来源：中国科学院大学
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