Isolation of high-purity casticin from artemisia annua l. by high-speed counter-current chromatography
文献类型：期刊论文
| 作者 | Han, Xiao; Ma, Xiaofeng; Zhang, Tianyou; Zhang, Yabin; Liu, Qinghui; Ito, Yoichiro |
| 刊名 | Journal of chromatography a
 |
| 出版日期 | 2007-06-01 |
| 卷号 | 1151期号:1-2页码:180-182 |
| 关键词 | Counter-current chromatography Preparative chromatography Artemisia annua l. Casticin |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2007.02.105 |
| 通讯作者 | Han, xiao(ssisyhan@yahoo.com.cn) |
| 英文摘要 | Following an initial clean-up step on silica, high-speed counter-current chromatography (hsccc) was used to purify a flavone, casticin (5,3 '-dihydroxy-3,6,7, 4 '-tetramethoxyflavone), from an extract of the dried leaves of artemisia annua l. the two-phase solvent system used was composed of n-hexane-ethyl acetate-methanol-water at an optimized volume ratio of 7:10:7:10 (v/v). hsccc separation of 226.4 mg of crude sample (containing casticin at 16.5% purity after silica gel clean-up) yielded 36.3 mg of casticin with a purity of over 99% and 96.2% recovery. identification of the target compound was performed by h-1 nmr, c-13 nmr, two-dimensional nmr, electrospray ionization ms, ir and uv. (c) 2007 elsevier b.v. all rights reserved. |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000247049400030 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2381098 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Han, Xiao |
| 作者单位 | 1.Beijing UE Biotech Co Ltd, Chinese Key Lab Natl Reference Mat Nat Prod, Beijing 100035, Peoples R China 2.Grad Univ Chinese Acad Sci, Dept Biol, Beijing 100049, Peoples R China 3.NHLBI, Ctr Biochem & Biophys, NIH, Bethesda, MD 20892 USA |
| 推荐引用方式 GB/T 7714 | Han, Xiao,Ma, Xiaofeng,Zhang, Tianyou,et al. Isolation of high-purity casticin from artemisia annua l. by high-speed counter-current chromatography[J]. Journal of chromatography a,2007,1151(1-2):180-182. |
| APA | Han, Xiao,Ma, Xiaofeng,Zhang, Tianyou,Zhang, Yabin,Liu, Qinghui,&Ito, Yoichiro.(2007).Isolation of high-purity casticin from artemisia annua l. by high-speed counter-current chromatography.Journal of chromatography a,1151(1-2),180-182. |
| MLA | Han, Xiao,et al."Isolation of high-purity casticin from artemisia annua l. by high-speed counter-current chromatography".Journal of chromatography a 1151.1-2(2007):180-182. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。