Covalent immobilization of glucose oxidase onto poly(styrene-co-glycidyl methacrylate) monodisperse fluorescent microspheres synthesized by dispersion polymerization
文献类型：期刊论文
| 作者 | Hou, Xiaohui; Liu, Bailing; Deng, Xiaobo; Zhang, Baotan; Chen, Hualin; Luo, Rong |
| 刊名 | Analytical biochemistry
 |
| 出版日期 | 2007-09-01 |
| 卷号 | 368期号:1页码:100-110 |
| 关键词 | Fluorescein Monodisperse microspheres Spectrophotometer Covalent immobilization Conformational changes Enzyme immobilization |
| ISSN号 | 0003-2697 |
| DOI | 10.1016/j.ab.2007.04.034 |
| 通讯作者 | Liu, bailing(blliuchem@hotmail.com) |
| 英文摘要 | In this study, micron-sized poly(styrene-co-glycidyl methacrylate) (pst-gma) fluorescent microspheres of 5.1 put in diameter were synthesized via dispersion polymerization of styrene and glycidyl methacrylate in the presence of 1,4-bis(5-phenyloxazol-2-yl) benzene (popop), which provided surface functional groups for covalent immobilization of enzymes. in an effort to study the biocompatibility of the microspheres' surface, glucose oxidase and beta-d-(+)-glucose were selected as a catalytic system for enzymatic assays. a colorimetric method was adopted in evaluating enzymatic activity by introducing horseradish peroxidase (hrp). both the immobilization amount and the apparent activity of immobilized glucose oxidase from asj)ergillitsniger (god) were determined at different conditions. the results show that the immobilized enzymes retained approximately 28 to 34% activity, as compared with free enzymes, without pronounced alteration of the optimum ph and temperature. kinetics studies show that the corresponding values of k-m and v-max are 23.2944 mm and 21.6450 m/min center dot mg god for free enzymes and 35.1780 mm and 15.4799 m/min center dot mg god for immobilized enzymes. the operational stability studies show that immobilized god could retain nearly 50% initial activity after being washed 20 times. the results suggest that the resultant pst-gma fluorescent microspheres provide a suitable surface for covalent immobilizing biomolecules; therefore, they have the potential of being used in fluorescence-based immunoassays in high-throughput screening or biosensors. (c) 2007 elsevier inc. all rights reserved. |
| WOS关键词 | CONFORMATIONAL-CHANGES ; SOLID SUPPORTS ; CROSS-LINKING ; PROTEIN ; IMMUNOASSAYS ; ADSORPTION ; ENZYMES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000248364400012 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2381258 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liu, Bailing |
| 作者单位 | 1.Chinese Acad Sci, Chengdu Inst Organ Chem, Chengdu 610041, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hou, Xiaohui,Liu, Bailing,Deng, Xiaobo,et al. Covalent immobilization of glucose oxidase onto poly(styrene-co-glycidyl methacrylate) monodisperse fluorescent microspheres synthesized by dispersion polymerization[J]. Analytical biochemistry,2007,368(1):100-110. |
| APA | Hou, Xiaohui,Liu, Bailing,Deng, Xiaobo,Zhang, Baotan,Chen, Hualin,&Luo, Rong.(2007).Covalent immobilization of glucose oxidase onto poly(styrene-co-glycidyl methacrylate) monodisperse fluorescent microspheres synthesized by dispersion polymerization.Analytical biochemistry,368(1),100-110. |
| MLA | Hou, Xiaohui,et al."Covalent immobilization of glucose oxidase onto poly(styrene-co-glycidyl methacrylate) monodisperse fluorescent microspheres synthesized by dispersion polymerization".Analytical biochemistry 368.1(2007):100-110. |
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来源：中国科学院大学
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