Cloning, expression and characterization of a nucleoside diphosphate kinase (ndpk) gene from tobacco
文献类型：期刊论文
| 作者 | Zhou, Qiyun; Xie, Zongming; Zhang, Zhigang; Cao, Yangrong; Zhang, Jinsong; Chen, Shouyi |
| 刊名 | Progress in natural science
 |
| 出版日期 | 2007-08-01 |
| 卷号 | 17期号:8页码:906-912 |
| 关键词 | Abiotic stress Autophosphorylation activity Ntndpki Tobacco Yeast two-hybrid |
| ISSN号 | 1002-0071 |
| 通讯作者 | Chen, shouyi(sychen@genetics.ac.cn) |
| 英文摘要 | Nucleoside diphosphate kinase (ndpk) is a housekeeping enzyme that maintains the intracellular levels of all (d)ntps used in biosynthesis except atp. here we report that a full-length cdna encoding nucleoside diphosphate kinase (ndpk) was cloned using yeast two-hybrid approach. a tobacco ndpk gene was obtained and designated as ntndpk1. ntndpk1 is 704 bp in length and encodes a putative 16.2 kd protein of 148 amino acids. phylogenic analysis showed that ntndpk1 is highly homologous to other plant ndpk genes and identified as type i (ndpk1). rna-gel blot analysis showed that there was no significant difference of ntndpk1 expression in roots, stems, leaves and buds. and expression of ntndpk1 was induced by aba and peg and repressed by nacl, but not significantly affected by paraquat, wounding and low temperature (4 degrees c) treatments, indicating that ntndpk1 may play a certain role in response to abiotic stress. in vitro phosphorylation assay demonstrated that ntndpk1 had autophosphorylation activity. |
| WOS关键词 | SIGNAL-TRANSDUCTION ; SALT STRESS ; ARABIDOPSIS ; ETHYLENE ; PROTEIN ; RICE ; HISTIDINE ; PLANTS ; AUTOPHOSPHORYLATION ; PHOSPHORYLATION |
| WOS研究方向 | Materials Science ; Science & Technology - Other Topics |
| WOS类目 | Materials Science, Multidisciplinary ; Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000249864400005 |
| 出版者 | TAYLOR & FRANCIS LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2381541 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chen, Shouyi |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Qiyun,Xie, Zongming,Zhang, Zhigang,et al. Cloning, expression and characterization of a nucleoside diphosphate kinase (ndpk) gene from tobacco[J]. Progress in natural science,2007,17(8):906-912. |
| APA | Zhou, Qiyun,Xie, Zongming,Zhang, Zhigang,Cao, Yangrong,Zhang, Jinsong,&Chen, Shouyi.(2007).Cloning, expression and characterization of a nucleoside diphosphate kinase (ndpk) gene from tobacco.Progress in natural science,17(8),906-912. |
| MLA | Zhou, Qiyun,et al."Cloning, expression and characterization of a nucleoside diphosphate kinase (ndpk) gene from tobacco".Progress in natural science 17.8(2007):906-912. |
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来源：中国科学院大学
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