Integration of monolithic frit into the particulate capillary (IMFPC) column in shotgun proteome analysis
文献类型：期刊论文
| 作者 | Wang, Fangjun; Dong, Jing; Ye, Mingliang; Wu, Ren'an; Zou, Hanfa |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2009-10-12 |
| 卷号 | 652期号:1-2页码:324-330 |
| 关键词 | Monolithic frit Nano-flow liquid chromatography Particulate column Fritless column Mouse liver |
| ISSN号 | 0003-2670 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | capillary column plays an important role in nano-flow liquid chromatography coupled with tandem mass spectrometry for dealing with the high dynamic range and complexity of protein samples in shotgun proteome analysis. in this study, the integrated monolithic frit into the particulate capillary (imfpc) column was prepared. by comparing the prepared imfpc column with conventionally fritless capillary column, smaller size of packing materials could be easily packed into the capillary to achieve higher average peak capacity and proteome coverage. as the monolithic emitter was integrated onto this type of column, the void volume between packing particles and electrospray emitter was eliminated and the electrospray quality was improved. the prepared imfpc column was applied to proteome analysis of mouse liver extracts, and it was observed that the number of identified proteins and peptides increased 14.9% and 12.9% as well as the peak capacity increased 11.6% by using imfpc column over conventionally fritless capillary column. (c) 2009 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | electrospray mass-spectrometry ; liquid-chromatography ; nanoelectrospray ionization ; multidimensional chromatography ; identification technology ; stationary phases ; ms/ms ; electrochromatography ; separation ; peptides |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000270759800034 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/101817]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Fangjun,Dong, Jing,Ye, Mingliang,et al. Integration of monolithic frit into the particulate capillary (IMFPC) column in shotgun proteome analysis[J]. analytica chimica acta,2009,652(1-2):324-330. |
| APA | Wang, Fangjun,Dong, Jing,Ye, Mingliang,Wu, Ren'an,&Zou, Hanfa.(2009).Integration of monolithic frit into the particulate capillary (IMFPC) column in shotgun proteome analysis.analytica chimica acta,652(1-2),324-330. |
| MLA | Wang, Fangjun,et al."Integration of monolithic frit into the particulate capillary (IMFPC) column in shotgun proteome analysis".analytica chimica acta 652.1-2(2009):324-330. |
入库方式： OAI收割
来源：大连化学物理研究所
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