Label free electrochemiluminescence protocol for sensitive dna detection with a tris(2,2'-bipyridyl)ruthenium(ii) modified electrode based on nucleic acid oxidation
文献类型：期刊论文
| 作者 | Wei, Hui; Du, Yan; Kang, Jianzhen; Wang, Erkang |
| 刊名 | Electrochemistry communications
 |
| 出版日期 | 2007-07-01 |
| 卷号 | 9期号:7页码:1474-1479 |
| 关键词 | Electrochemiluminescence Dna sensors Label free Single base mismatch |
| ISSN号 | 1388-2481 |
| DOI | 10.1016/j.elecom.2007.01.053 |
| 通讯作者 | Wang, erkang(ekwang@ciac.jl.cn) |
| 英文摘要 | Label free electrochemiluminescence (ecl) dna detection based on catalytic guanine and adenine bases oxidation using tris(2,2'-bipyridyl)ruthenium(ii) [ru(bpy)(3)(2+)] modified glassy carbon (gc) electrode was demonstrated in this work. the modified gc electrode was prepared by casting carbon nanotubes (cnt)/nafion/ru(bpy)(3)(2+) composite film on the electrode surface. ecl signals of doublestranded dna and their thermally denatured counterparts can be distinctly discriminated using cyclic voltammetry (cv) with a low concentration (3.04 x 10(-8) mol/l for salmon testes-dna). most importantly, sensitive single-base mismatch detection of p53 gene sequence segment was realized with 3.93 x 10(-10) mol/l employing cv stimulation (ecl signal of c/a mismatched dna oligonucleotides was 1.5-fold higher than that of fully base-paired dna oligonucleotides). label free, high sensitivity and simplicity for single-base mismatch discrimination were the main advantages of the present ecl technique for dna detection over the traditional dna sensors. (c) 2007 elsevier b.v. all rights reserved. |
| WOS关键词 | ELECTROGENERATED CHEMILUMINESCENCE ; GUANINE OXIDATION ; ULTRATHIN FILMS ; RU(BPY)(3)(2+) ; RUTHENIUM(II) ; NANOPARTICLES ; HYBRIDIZATION ; ISOTOPE ; SOLVENT ; TARGETS |
| WOS研究方向 | Electrochemistry |
| WOS类目 | Electrochemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000248089400010 |
| 出版者 | ELSEVIER SCIENCE INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2381584 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Erkang |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Hui,Du, Yan,Kang, Jianzhen,et al. Label free electrochemiluminescence protocol for sensitive dna detection with a tris(2,2'-bipyridyl)ruthenium(ii) modified electrode based on nucleic acid oxidation[J]. Electrochemistry communications,2007,9(7):1474-1479. |
| APA | Wei, Hui,Du, Yan,Kang, Jianzhen,&Wang, Erkang.(2007).Label free electrochemiluminescence protocol for sensitive dna detection with a tris(2,2'-bipyridyl)ruthenium(ii) modified electrode based on nucleic acid oxidation.Electrochemistry communications,9(7),1474-1479. |
| MLA | Wei, Hui,et al."Label free electrochemiluminescence protocol for sensitive dna detection with a tris(2,2'-bipyridyl)ruthenium(ii) modified electrode based on nucleic acid oxidation".Electrochemistry communications 9.7(2007):1474-1479. |
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来源：中国科学院大学
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