中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Characterization and fine mapping of the ibf mutant in rice

文献类型:期刊论文

作者Cui, Jiajun; Fan, Shengci; Shao, Tian; Huang, Zejun; Zheng, Dali; Tang, Ding; Li, Ming; Qian, Qian; Cheng, Zhukuan
刊名Journal of integrative plant biology
出版日期2007-05-01
卷号49期号:5页码:678-685
关键词Brown furrows Fine mapping Ibf mutant Oryza sativa
ISSN号1672-9072
DOI10.1111/j.1672-9072.2007.00467.x
通讯作者Cheng, zhukuan(zkcheng@genetics.ac.cn)
英文摘要The pigment is an important character in plant development. in the present study, we characterized and fine mapped one inhibitor for brown furrows gene (ibf in rice (oryza sativa l.). in the ibf mutant, brown pigments specifically accumulate in the furrows of hulls as seeds mature and reach a maximum level in dry seeds. genetic analysis showed that the mutant phenotype is controlled by one recessive nuclear gene, which was finally mapped in a 90-kb region on the long arm of chromosome 9. polymerase chain reaction and southern blotting analysis revealed that there was a 26 kb deletion in the 90-kb region in the mutant. since all the open reading frames outside the gap in the delimited region had no detectable difference in dna sequence with the wild-type, we postulated that the ibf locus should be located in the gap. through gene annotation and reverse transcription-polymerase chain reaction (rt-pcr) analysis, we selected oskf1 encoding a kelch repeat-containing f-box family protein as the candidate gene of w.
WOS关键词SEED COAT ; FLAVONOID BIOSYNTHESIS ; GENE ; PROTEIN ; ENCODES ; MAIZE ; PERICARP-COLOR1 ; BIOCHEMISTRY ; SEQUENCE ; ALLELES
WOS研究方向Biochemistry & Molecular Biology ; Plant Sciences
WOS类目Biochemistry & Molecular Biology ; Plant Sciences
语种英语
WOS记录号WOS:000246304000016
出版者WILEY-BLACKWELL
URI标识http://www.irgrid.ac.cn/handle/1471x/2381589
专题中国科学院大学
通讯作者Cheng, Zhukuan
作者单位1.China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Peoples R China
2.Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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GB/T 7714
Cui, Jiajun,Fan, Shengci,Shao, Tian,et al. Characterization and fine mapping of the ibf mutant in rice[J]. Journal of integrative plant biology,2007,49(5):678-685.
APA Cui, Jiajun.,Fan, Shengci.,Shao, Tian.,Huang, Zejun.,Zheng, Dali.,...&Cheng, Zhukuan.(2007).Characterization and fine mapping of the ibf mutant in rice.Journal of integrative plant biology,49(5),678-685.
MLA Cui, Jiajun,et al."Characterization and fine mapping of the ibf mutant in rice".Journal of integrative plant biology 49.5(2007):678-685.

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来源:中国科学院大学

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