Characterization and fine mapping of the ibf mutant in rice
文献类型:期刊论文
作者 | Cui, Jiajun; Fan, Shengci; Shao, Tian; Huang, Zejun; Zheng, Dali; Tang, Ding; Li, Ming; Qian, Qian; Cheng, Zhukuan |
刊名 | Journal of integrative plant biology
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出版日期 | 2007-05-01 |
卷号 | 49期号:5页码:678-685 |
关键词 | Brown furrows Fine mapping Ibf mutant Oryza sativa |
ISSN号 | 1672-9072 |
DOI | 10.1111/j.1672-9072.2007.00467.x |
通讯作者 | Cheng, zhukuan(zkcheng@genetics.ac.cn) |
英文摘要 | The pigment is an important character in plant development. in the present study, we characterized and fine mapped one inhibitor for brown furrows gene (ibf in rice (oryza sativa l.). in the ibf mutant, brown pigments specifically accumulate in the furrows of hulls as seeds mature and reach a maximum level in dry seeds. genetic analysis showed that the mutant phenotype is controlled by one recessive nuclear gene, which was finally mapped in a 90-kb region on the long arm of chromosome 9. polymerase chain reaction and southern blotting analysis revealed that there was a 26 kb deletion in the 90-kb region in the mutant. since all the open reading frames outside the gap in the delimited region had no detectable difference in dna sequence with the wild-type, we postulated that the ibf locus should be located in the gap. through gene annotation and reverse transcription-polymerase chain reaction (rt-pcr) analysis, we selected oskf1 encoding a kelch repeat-containing f-box family protein as the candidate gene of w. |
WOS关键词 | SEED COAT ; FLAVONOID BIOSYNTHESIS ; GENE ; PROTEIN ; ENCODES ; MAIZE ; PERICARP-COLOR1 ; BIOCHEMISTRY ; SEQUENCE ; ALLELES |
WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
WOS类目 | Biochemistry & Molecular Biology ; Plant Sciences |
语种 | 英语 |
WOS记录号 | WOS:000246304000016 |
出版者 | WILEY-BLACKWELL |
URI标识 | http://www.irgrid.ac.cn/handle/1471x/2381589 |
专题 | 中国科学院大学 |
通讯作者 | Cheng, Zhukuan |
作者单位 | 1.China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Peoples R China 2.Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
推荐引用方式 GB/T 7714 | Cui, Jiajun,Fan, Shengci,Shao, Tian,et al. Characterization and fine mapping of the ibf mutant in rice[J]. Journal of integrative plant biology,2007,49(5):678-685. |
APA | Cui, Jiajun.,Fan, Shengci.,Shao, Tian.,Huang, Zejun.,Zheng, Dali.,...&Cheng, Zhukuan.(2007).Characterization and fine mapping of the ibf mutant in rice.Journal of integrative plant biology,49(5),678-685. |
MLA | Cui, Jiajun,et al."Characterization and fine mapping of the ibf mutant in rice".Journal of integrative plant biology 49.5(2007):678-685. |
入库方式: iSwitch采集
来源:中国科学院大学
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