Mesoporous silica hollow sphere (MSHS) for the bioelectrochemistry of horseradish peroxidase
文献类型：期刊论文
| 作者 | Cao, Zhaoxia1,2; Zhang, Jian1,2; Zeng, Julan1,3; Sun, Lixian1; Xu, Fen1; Cao, Zhong3; Zhang, Ling3; Yang, Daowu3 |
| 刊名 | talanta
 |
| 出版日期 | 2009-01-15 |
| 卷号 | 77期号:3页码:943-947 |
| 关键词 | Hollow mesoporous silica spheres Horseradish peroxidase Biosensor Direct electron transfer |
| 产权排序 | 1;1 |
| 通讯作者 | 孙立贤 |
| 英文摘要 | in this work, novel mesoporous silica hollow spheres (mshs) were chosen as an immobilization matrix, to construct a mediator-free third-generation hrp biosensor. uv-vis spectroscopy revealed that horseradish peroxidase (hrp) entrapped in mshs could retain its native structure. ftir spectroscopy and nitrogen adsorption-desorption isotherms indicated that hrp are intercalated into the mesopores. the direct electron transfer of hrp entrapped in mshs was observed. a pair of stable and well-defined redox peaks of hrp with a formal potential of about -0.150v (vs. ag/agcl) in 0.1 m ph 7.0 phosphate-buffered solution (pbs) were obtained. the biosensor exhibited a fast amperometric response to h(2)o(2) with a linear range of 3.9 x 10(-6) to 1.4 x 10(-4) m (r = 0.997, n = 20). the detection limit was 1.2 x 10(-6) m based s/n = 3. (c) 2008 published by elsevier b.v. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | direct electrochemistry ; hydrogen-peroxide ; enzyme immobilization ; lysozyme adsorption ; catalytic activity ; h2o2 biosensor ; gold electrode ; matrix ; nanoparticles ; encapsulation |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000262761300002 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/101849]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Mat &Thermochem Lab, Dalian 116023, Liaoning Prov, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Changsha Univ Sci & Technol, Sch Chem & Environm Engn, Changsha 410076, Hunan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Zhaoxia,Zhang, Jian,Zeng, Julan,et al. Mesoporous silica hollow sphere (MSHS) for the bioelectrochemistry of horseradish peroxidase[J]. talanta,2009,77(3):943-947. |
| APA | Cao, Zhaoxia.,Zhang, Jian.,Zeng, Julan.,Sun, Lixian.,Xu, Fen.,...&Yang, Daowu.(2009).Mesoporous silica hollow sphere (MSHS) for the bioelectrochemistry of horseradish peroxidase.talanta,77(3),943-947. |
| MLA | Cao, Zhaoxia,et al."Mesoporous silica hollow sphere (MSHS) for the bioelectrochemistry of horseradish peroxidase".talanta 77.3(2009):943-947. |
入库方式： OAI收割
来源：大连化学物理研究所
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