Direct electrochemistry and enhanced electrocatalysis of horseradish peroxidase based on flowerlike ZnO-gold nanoparticle-Nafion nanocomposite
文献类型：期刊论文
| 作者 | Xiang, Cuili2; Zou, Yongjin2; Sun, Li-Xian1; Xu, Fen3 |
| 刊名 | sensors and actuators b-chemical
 |
| 出版日期 | 2009-02-02 |
| 卷号 | 136期号:1页码:158-162 |
| 关键词 | Horseradish peroixdase Flowerlike ZnO Gold nanoparticles Direct electron transfer Biosensor |
| 产权排序 | 1;1 |
| 通讯作者 | 孙立贤 ; 徐芬 |
| 英文摘要 | we developed a new flowerlike zno-gold nanoparticles (gnps)-nafion nanocomposite which can promote the direct electron transfer of horseradish peroxidase (hrp) immobilized in the film effectively. the cyclic voltammetric results show that hrp entrapped in the composite could undergo a direct quasi-reversible electrochemical reaction with a formal potential (e-0') of -0.32v (versus ag/agcl) in phosphate buffer solution (pbs). the flowerlike zno and gnps show synergistic effect and the zno-gnps-nafion-hrp modified gc electrode gives an enhanced electrocatalytic activity towards the reduction of hydrogen peroxide (h2o2). the calculated apparent michaelis-menten constant (k-m(app)) is m 1.76 mm. which is much lower than that reported, indicating a high catalytic activity of hrp. the catalysis currents increase linearly to the h2o2 concentration in a wide range of 1.5 x 10(-5) to 1.1 x 10(-3) m, with a correlation coefficient of 0.998. the detection limit is 9.0 x 10(-6) m (at the ratio of signal to noise, s/n = 3), demonstrating that the formed film provides a favorable microenvironment for the enzyme to retain its activity. moreover, the modified electrode displays rapid response to h2o2 and possesses good stability and reproducibility. (c) 2008 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences ; technology |
| 类目[WOS] | chemistry, analytical ; electrochemistry ; instruments & instrumentation |
| 研究领域[WOS] | chemistry ; electrochemistry ; instruments & instrumentation |
| 关键词[WOS] | direct electron-transfer ; glassy-carbon electrode ; sol-gel ; biosensor ; hemoglobin ; nanotubes ; film ; immobilization ; composites ; tyrosinase |
| 收录类别 | SCI |
| 原文出处 | false |
| 语种 | 英语 |
| WOS记录号 | WOS:000263494200025 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/101855]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Mat & Thermochem Lab, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Liaoning Normal Univ, Coll Chem & Chem Engn, Dalian 116029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiang, Cuili,Zou, Yongjin,Sun, Li-Xian,et al. Direct electrochemistry and enhanced electrocatalysis of horseradish peroxidase based on flowerlike ZnO-gold nanoparticle-Nafion nanocomposite[J]. sensors and actuators b-chemical,2009,136(1):158-162. |
| APA | Xiang, Cuili,Zou, Yongjin,Sun, Li-Xian,&Xu, Fen.(2009).Direct electrochemistry and enhanced electrocatalysis of horseradish peroxidase based on flowerlike ZnO-gold nanoparticle-Nafion nanocomposite.sensors and actuators b-chemical,136(1),158-162. |
| MLA | Xiang, Cuili,et al."Direct electrochemistry and enhanced electrocatalysis of horseradish peroxidase based on flowerlike ZnO-gold nanoparticle-Nafion nanocomposite".sensors and actuators b-chemical 136.1(2009):158-162. |
入库方式： OAI收割
来源：大连化学物理研究所
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